BEN domain explained

Symbol:	BEN
BEN
Pfam:	PF10523
Interpro:	IPR018379

In molecular biology, the BEN domain is a protein domain which is found in diverse proteins including:

• SMAR1 (Scaffold/Matrix attachment region-binding protein 1; also known as BANP), a tumour-suppressor MAR-binding protein that down-regulates Cyclin D1 expression by recruiting HDAC1-mSin3A co-repressor complex at Cyclin D1 promoter locus; SMAR1 is the target of prostaglandin A2 (PGA2) induced growth arrest.^{[1] [2]}
• NACC1, a novel member of the POZ/BTB (Pox virus and Zinc finger/Broad complex, Tramtrack and Bric-a-brac), but which varies from other proteins of this class in that it lacks the characteristic DNA-binding motif.^[3]
• Mod(mdg4) isoform C, the modifier of the mdg4 locus in Drosophila melanogaster (Fruit fly), where mdg4 encodes chromatin proteins which are involved in position effect variegation, establishment of chromatin boundaries, nerve path finding, meiotic chromosome pairing and apoptosis.^[4] Trans-splicing of Mod(mdg4) produces at least 26 transcripts.
• BEND2, a protein of unknown function, that is predicted to be involved in chromatin modification and has been associated clinically with central nervous system disorders.
• E5R protein from Chordopoxvirus virosomes, which is found in cytoplasmic sites of viral DNA replication.^[5]
• Several proteins of polydnaviruses.

The BEN domain is predicted to function as an adaptor for the higher-order structuring of chromatin, and recruitment of chromatin modifying factors in transcriptional regulation. It has been suggested to mediate protein-DNA and protein-protein interactions during chromatin organization and transcription. The presence of BEN domains in a poxviral early virosomal protein and in polydnaviral proteins also suggests a possible role in the organisation of viral DNA during replication or transcription. They are generally linked to other globular domains with functions related to transcriptional regulation and chromatin structure, such as BTB, C4DM, and C2H2 fingers.^[6]

This domain is predicted to form an all-alpha fold with four conserved helices. Its conservation pattern revealed several conserved residues, most of which have hydrophobic side-chains and are likely to stabilize the fold through helix-helix packing.^[6] First human BEN domain (BEND3)structure is solved together with TPR (ERCC6L)domain and Stimulates the ERCC6L translocase and ATPase activities.^[7]

Further reading

• Zheng . Luqian . Liu . Jingjing . Niu . Lijie . Kamran . Mohammad . Yang . Ally W.H. . Jolma . Arttu . Dai . Qi . Hughes . Timothy R. . Patel . Dinshaw J. . Zhang . Long . Prasanth . Supriya G. . Yu . Yang . Ren . Aiming . Lai . Eric C. . 5. Distinct structural bases for sequence-specific DNA binding by mammalian BEN domain proteins . Genes & Development . Cold Spring Harbor Laboratory . 2022-02-10 . 0890-9369 . 10.1101/gad.348993.121 . free . 8887127 .

Notes and References

1. Rampalli S, Pavithra L, Bhatt A, Kundu TK, Chattopadhyay S . Tumor suppressor SMAR1 mediates cyclin D1 repression by recruitment of the SIN3/histone deacetylase 1 complex . Molecular and Cellular Biology . 25 . 19 . 8415–29 . October 2005 . 16166625 . 1265755 . 10.1128/MCB.25.19.8415-8429.2005 .
2. Pavithra L, Rampalli S, Sinha S, Sreenath K, Pestell RG, Chattopadhyay S . Stabilization of SMAR1 mRNA by PGA2 involves a stem loop structure in the 5' UTR . Nucleic Acids Research . 35 . 18 . 6004–16 . 2007 . 17726044 . 2094063 . 10.1093/nar/gkm649 .
3. Mackler S, Pacchioni A, Degnan R, Homan Y, Conti AC, Kalivas P, Blendy JA . Requirement for the POZ/BTB protein NAC1 in acute but not chronic psychomotor stimulant response . Behavioural Brain Research . 187 . 1 . 48–55 . February 2008 . 17945361 . 2248375 . 10.1016/j.bbr.2007.08.036 .
4. Krauss V, Dorn R . Evolution of the trans-splicing Drosophila locus mod(mdg4) in several species of Diptera and Lepidoptera . Gene . 331 . 165–76 . April 2004 . 15094203 . 10.1016/j.gene.2004.02.019 .
5. Murcia-Nicolas A, Bolbach G, Blais JC, Beaud G . Identification by mass spectroscopy of three major early proteins associated with virosomes in vaccinia virus-infected cells . Virus Research . 59 . 1 . 1–12 . January 1999 . 10854161 . 10.1016/S0168-1702(98)00114-2.
6. Abhiman S, Iyer LM, Aravind L . BEN: a novel domain in chromatin factors and DNA viral proteins . Bioinformatics . 24 . 4 . 458–61 . February 2008 . 18203771 . 2477736 . 10.1093/bioinformatics/btn007 .
7. Pitchai . Ganesha P. . Kaulich . Manuel . Bizard . Anna H. . Mesa . Pablo . Yao . Qi . Sarlos . Kata . Streicher . Werner W. . Nigg . Erich A. . Montoya . Guillermo . Hickson . Ian D. . A novel TPR–BEN domain interaction mediates PICH–BEND3 association . Nucleic Acids Research . 2017-11-02 . 45 . 19 . 11413–11424 . 10.1093/nar/gkx792. 5737856 .