B5 protein domain explained

Symbol:	B5
B5 protein domain
Pfam:	PF03484
Interpro:	IPR005147
Prosite:	PDOC00464
Scop:	1qq3
Tcdb:	5.A.4

In molecular biology, Domain B5 is found in phenylalanine-tRNA synthetase beta subunits. This domain has been shown to bind DNA through a winged helix-turn-helix motif.^[1] Phenylalanine-tRNA synthetase may influence common cellular processes via DNA binding, in addition to its aminoacylation function.

Function

The beta domain, in particular, B3/B4, is required for the correct amino acid to be joined to the corresponding tRNA. Hence, the B3/B4 domain is crucial to accurate translation. Failure to do so, results in a mutated protein which improperly folds and consequently protein function is affected.^[2] /B

Notes and References

1. Dou X, Limmer S, Kreutzer R . DNA-binding of phenylalanyl-tRNA synthetase is accompanied by loop formation of the double-stranded DNA . J. Mol. Biol. . 305 . 3 . 451–8 . January 2001 . 11152603 . 10.1006/jmbi.2000.4312 .
2. Roy H, Ling J, Irnov M, Ibba M . Post-transfer editing in vitro and in vivo by the beta subunit of phenylalanyl-tRNA synthetase. . EMBO J . 2004 . 23 . 23 . 4639–48 . 15526031 . 10.1038/sj.emboj.7600474 . 533057 .