| Official Name: | Ayene |
| Pushpin Map: | Equatorial Guinea mainland |
| Pushpin Mapsize: | 300 |
| Pushpin Label Position: | bottom |
| Pushpin Map Caption: | Location in Equatorial Guinea |
| Subdivision Type: | Country |
| Subdivision Name: |  Equatorial Guinea |
| Subdivision Name1: | Wele-Nzas |
| Subdivision Type2: | District |
| Subdivision Name2: | Añisok |
| Population As Of: | 2005 |
| Population Total: | 3482 |
| Population Blank1 Title: | Ethnicities |
| Population Blank2 Title: | Religions |
| Coordinates: | 1.9089°N 10.6353°W |
Ayene is a town in Equatorial Guinea. It is located in the Añisok district in the province of Wele-Nzas and has a (2005 est.) population of 3,482.