See main article: Athletics at the 2010 South American Games. The Women's 4x100m Relay event at the 2010 South American Games was held on March 22 at 18:25.
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Vanusa dos Santos Vanda Gomes Ana Claudia Silva Franciela Krasucki | Nelcy Caicedo Yenifer Padilla Maria Alejandra Idrobo Yomara Hinestroza | María Ayelén Diogo María Florencia Lamboglia Constanza Eckhardt Agustina Zerboni |
| Standing records prior to the 2010 South American Games | |||||
|---|---|---|---|---|---|
| World record | 41.37 | Canberra, Australia | 6 October 1985 | ||
| World Leading | 44.46 | Kingston, Jamaica | 27 February 2010 | ||
| South American record | 42.97 | Bogotá, Colombia | 10 July 2004 | ||
| South American U23 record | 43.98 | São Paulo, Brazil | 7 July 2007 | ||
Results were published.
| Rank | width=150 | Athlete ! | width=100 | Nationality ! | width=100 | Reaction ! | width=100 | Result ! | Notes |
|---|---|---|---|---|---|---|---|---|---|
| 0.204 | 44.47 | ||||||||
| Nelcy Caicedo Yenifer Padilla Maria Alejandra Idrobo Yomara Hinestroza | 0.231 | 44.94 | |||||||
| María Ayelén Diogo María Florencia Lamboglia Constanza Eckhardt Agustina Zerboni | 0.199 | 46.76 | |||||||
| 4 | Carolina Castillo Ljubica Milos Anneliese Grosser Isidora Jiménez | 47.29 | |||||||