See main article: Athletics at the 2010 South American Games. The Men's 4x400m Relay event at the 2010 South American Games was held on March 23 at 19:15.
| width=200 bgcolor=gold | Gold | width=200 bgcolor=silver | Silver | width=200 bgcolor=CC9966 | Bronze |
| Rubén Headly Georni Jaramillo Arturo Ramírez Omar Longart | José Guilherme de Oliveira Hederson Estefani Henrique de Souza Hélder Alves | Juan Pablo Maturana Brayan Ambuila Javier Palacios Rafith Rodríguez |
| Standing records prior to the 2010 South American Games | |||||
|---|---|---|---|---|---|
| World record | 2:54.29 | Stuttgart, Germany | 22 July 1993 | ||
| World Leading | 3:05.33 | Kingston, Jamaica | 27 February 2010 | ||
| South American record | 2:58.56 | Winnipeg, Canada | 30 July 1999 | ||
| South American U23 record | 3:04.88 | Caracas, Venezuela | 29 July 2001 | ||
Results were published.
| Rank | width=150 | Athlete ! | width=100 | Nationality ! | width=100 | Reaction ! | width=100 | Result ! | Notes |
|---|---|---|---|---|---|---|---|---|---|
| 0.217 | 3:06.53 | ||||||||
| 0.247 | 3:07.11 | ||||||||
| 0.200 | 3:09.03 | ||||||||
| 4 | 0.403 | 3:15.60 | |||||||
| 5 | 0.274 | 3:16.31 | |||||||
| 6 | 0.178 | 3:19.21 | |||||||
| – |  Perú | DNS |