The women's 4 × 100 metres relay event at the 2009 Summer Universiade was held on 11–12 July.
| width=200 valign=top | Audrey Alloh Doris Tomasini Giulia Arcioni Maria Aurora Salvagno | width=200 valign=top | Ewelina Ptak Marika Popowicz Dorota Jędrusińska Marta Jeschke Iwona Brzezińska | width=200 valign=top | Laetitia Denis Ayodelé Ikuesan Amandine Elard Lucienne M'belu |
* Athletes who competed in heats only and received medals.
Qualification: First 3 teams of each heat (Q) plus the next 2 fastest (q) qualified for the final.
| Rank | Heat | Nation | Athletes | Time | Notes | |
|---|---|---|---|---|---|---|
| 1 | 1 | 44.19 | Q | |||
| 2 | 2 | 44.46 | Q | |||
| 3 | 1 | 44.70 | Q | |||
| 4 | 2 | 44.73 | Q, SB | |||
| 5 | 1 | 44.89 | Q | |||
| 6 | 1 | 44.91 | q | |||
| 7 | 2 | 44.95 | Q | |||
| 8 | 1 | 45.29 | q, SB | |||
| 9 | 2 | 45.61 | ||||
| 2 | [1] | |||||
| 1 | ||||||
| 1 | ||||||
| 2 |
| Rank | Lane | Nation | Athletes | Time | Notes | |
|---|---|---|---|---|---|---|
| 4 | 43.83 | |||||
| 5 | 43.96 | |||||
| 8 | 44.31 | |||||
| 4 | 3 | 44.47 | ||||
| 5 | 6 | 44.48 | SB | |||
| 6 | 7 | 44.94 | ||||
| 7 | 1 | 45.01 | ||||
| 8 | 2 | 46.20 |