See main article: Athletics at the 2008 Summer Paralympics.
The Men's 400m T54 had its First Round held on September 8 at 10:05, its Semifinals on September 9 at 20:35 and its Final on September 10 at 21:00.
| Place | width=250 | Athlete ! | width=100 | Round 1 ! | width=100 | Semifinal ! | width=100 | Final | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 46.75 Q PR | 48.52 Q | 45.07 WR | ||||||||
| 2 | 47.26 Q | 47.46 Q | 46.02 | ||||||||
| 3 | 48.03 Q | 48.39 Q | 46.86 | ||||||||
| 4 | 48.99 Q | 50.01 q | 47.64 | ||||||||
| 5 | 47.78 Q | 49.40 q | 47.67 | ||||||||
| 6 | 49.32 Q | 48.75 Q | 48.39 | ||||||||
| 7 | 48.69 Q | 48.72 Q | 48.52 | ||||||||
| 8 | 49.37 Q | 51.09 Q | 49.20 | ||||||||
| 9 | 48.76 Q | 51.39 | |||||||||
| 10 | 49.75 Q | 51.66 | |||||||||
| 11 | 49.76 q | 51.67 | |||||||||
| 12 | 49.81 q | 51.69 | |||||||||
| 13 | 50.24 q | 52.84 | |||||||||
| 14 | 49.61 Q | 53.34 | |||||||||
| 15 | 49.05 Q | 54.46 | |||||||||
| 16 | 49.42 q | 58.75 | |||||||||
| 17 | 50.24 | ||||||||||
| 18 | 50.32 | ||||||||||
| 19 | 50.35 | ||||||||||
| 20 | 50.36 | ||||||||||
| 21 | 50.45 | ||||||||||
| 22 | 50.68 | ||||||||||
| 23 | 51.26 | ||||||||||
| 24 | 51.57 | ||||||||||
| 25 | 51.69 | ||||||||||
| 26 | 52.53 | ||||||||||
| DNS | |||||||||||