Asparaginyl-tRNA synthase (glutamine-hydrolysing) explained

asparaginyl-tRNA synthase (glutamine-hydrolyzing)
Ec Number:	6.3.5.6
Cas Number:	37211-76-0
Go Code:	0050566

In enzymology, an asparaginyl-tRNA synthase (glutamine-hydrolysing) is an enzyme that catalyzes the chemical reaction

ATP + aspartyl-tRNA^Asn + L-glutamine

\rightleftharpoons ADP + phosphate + asparaginyl-tRNA^Asn + L-glutamate

The 3 substrates of this enzyme are ATP, aspartyl-tRNA(Asn), and L-glutamine, whereas its 4 products are ADP, phosphate, asparaginyl-tRNA(Asn), and L-glutamate.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds carbon-nitrogen ligases with glutamine as amido-N-donor. The systematic name of this enzyme class is aspartyl-tRNAAsn:L-glutamine amido-ligase (ADP-forming). This enzyme participates in glutamate metabolism and alanine and aspartate metabolism.

References

• Min B, Pelaschier JT, Graham DE, Tumbula-Hansen D, Soll D . 2002 . Transfer RNA-dependent amino acid biosynthesis: an essential route to asparagine formation . Proc. Natl. Acad. Sci. U.S.A. . 99 . 2678–83 . 11880622 . 10.1073/pnas.012027399 . 5 . 122407 . 2002PNAS...99.2678M . free .
• Curnow AW, Tumbula DL, Pelaschier JT, Min B, Soll D . 1998 . Glutamyl-tRNA(Gln) amidotransferase in Deinococcus radiodurans may be confined to asparagine biosynthesis . Proc. Natl. Acad. Sci. U.S.A. . 95 . 12838–43 . 9789001 . 10.1073/pnas.95.22.12838 . 22 . 23620 . 1998PNAS...9512838C . free .
• Ibba M, Soll D . 2000 . Aminoacyl-tRNA synthesis . Annu. Rev. Biochem. . 69 . 1 . 617–50 . 10966471 . 10.1146/annurev.biochem.69.1.617 .