Archaeosine synthase explained

Archaeosine synthase
Ec Number:	2.6.1.97

Archaeosine synthase (ArcS, TgtA2, MJ1022 (gene), glutamine:preQ0-tRNA amidinotransferase) is an enzyme with systematic name L-glutamine:7-cyano-7-carbaguanine aminotransferase.^{[1] [2]} This enzyme catalyses the following chemical reaction

L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O

\rightleftharpoons L-glutamate + archaeine¹⁵ in tRNA

In Euryarchaeota the reaction is catalysed by ArcS.

Notes and References

1. Phillips G, Chikwana VM, Maxwell A, El-Yacoubi B, Swairjo MA, Iwata-Reuyl D, de Crécy-Lagard V . Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA . The Journal of Biological Chemistry . 285 . 17 . 12706–13 . April 2010 . 20129918 . 2857094 . 10.1074/jbc.M110.102236 . free .
2. Phillips G, Swairjo MA, Gaston KW, Bailly M, Limbach PA, Iwata-Reuyl D, de Crécy-Lagard V . Diversity of archaeosine synthesis in crenarchaeota . ACS Chemical Biology . 7 . 2 . 300–5 . February 2012 . 22032275 . 3289047 . 10.1021/cb200361w .