Apusomonadidae Explained

The apusomonads (family Apusomonadidae) are a group of protozoan zooflagellates that glide on surfaces, and mostly consume prokaryotes. They are of particular evolutionary interest because they appear to be the sister group to the Opisthokonts, the clade that includes both animals and fungi. Together with the Breviatea, these form the Obazoa clade.

Characteristics

Apusomonads are small gliding heterotrophic biflagellates (i.e. with two flagella) that possess a proboscis, formed partly or entirely by the anterior flagellum surrounded by a membranous sleeve. There is a pellicle under the dorsal cell membrane that extends into the proboscis sleeve and into a skirt that covers the sides of the cell. Apusomonads present two different cell plans:^[1]

• Derived cell plan, represented by Apusomonas, with a round cell body and a mastigophore, a projection of the cell containing both basal bodies at its end.^[1]
• "Amastigomonas-like" cell plan, with an oval or oblong cell that generally forms pseudopodia from the ventral surface, with no mastigophore, and the proboscis comprising solely the flagellum and the sleeve. These characteristics are considered 'primitive' or 'ancestral' in comparison with Apusomonas. Organisms with this body plan, although historically assigned to the same genus Amastigomonas, are a paraphyletic group from which Apusomonas has evolved.^[1]

Evolution

External relationships

The apusomonads are the sister group to Opisthokonta, the lineage that includes animals, fungi and an array of related protists. Because of this, apusomonads occupy an important phylogenetic position to understand eukaryotic evolution. They retain ancestral characteristics, such as the biflagellate body plan, which in opisthokonts evolves into a uniflagellate plan.

Apusomonads are vital to understanding multicellularity. Genes involved in multicellularity have been found in the apusomonad Thecamonas, such as adhesion proteins, calcium-signaling genes and types of sodium channels characteristic of animals.^[1] The genome of the strain "Amastigomonas sp." presents the integrin-mediated adhesion machinery, the primary cell-matrix adhesion mechanism seen in Metazoa (animals).

Internal relationships

Apusomonads are a poorly and narrowly studied group.^[1] Currently, the diversity of described apusomonads consists of the round Apusomonas and a wide array of "Amastigomonas-type" organisms that have been reclassified into the genera Thecamonas, Manchomonas, Podomonas, Multimonas, Chelonemonas and, most recently, Catacumbia, Cavaliersmithia, Karpovia, Mylnikovia and Singekia. The relationships between these genera are depicted by the cladogram below.

Taxonomy

History

Apusomonads were first described in 1989 as one family Apusomonadidae inside the monotypic order Apusomonadida, as a group of flagellates containing the genera Apusomonas and Amastigomonas. Later, British protozoologist Thomas Cavalier-Smith classified them within the monotypic class Thecomonadea as part of the paraphyletic phylum Apusozoa. Modern cladistic approaches to eukaryotic classification refer to apusomonads by their order-level name alone.^[2]

Classification

There are 10 recognized genera, as well as the "Amastigomonas-like" archetype that includes primitive forms not yet transferred to new genera.

• Amastigomonas
  • A. caudata [''Amastigomonas borokensis'' {{au|Hamar 1979}}]
  • A. debruynei
  • A. marisrubri
• Catacumbia
  • C. lutetiensis
• Cavaliersmithia
  • C. chaoae
• Multimonas
  • M. koreensis
  • M. marina [''Cercomonas marina'' {{au|Mylnikov 1989}}; ''Amastigomonas marina'' {{au|(Mylnikov 1989) Mylnikov 1999}}]
  • M. media
• Mylnikovia
  • M. oxoniensis [''Thecamonas oxoniensis'' {{au|Cavalier-Smith 2010}}]
• Podomonas
  • P. capensis
  • P. gigantea [''Amastigomonas gigantea'' {{au|Mylnikov 1999}}]
  • P. griebenis [''Amastigomonas griebenis'' {{au|Mylnikov 1999}}]
  • P. kaiyoae
  • P. klosteris [''Amastigomonas klosteris'' {{au|Arndt & Mylnikov 1999}}]
  • P. magna
• Apusomonadinae Cavalier-Smith 2010
  • Apusomonas [''[[Rostromonas]] ]
    • A. australiensis
    • A. proboscidea [''Rostromonas applanata'' {{au|Karpoff & Zhukov 1980}}]
  • Manchomonas
    • M. bermudensis [''Amastigomonas bermudensis'' {{au|Molina & Nerad 1991}}]
• Thecamonadinae [''Thecamonas''/''Chelomonas'' clade]
  • Chelonemonas
    • C. dolani
    • C. geobuk
    • C. masanensis
  • Karpovia
    • K. croatica
  • Singekia
    • S. franciliensis
    • S. montserratensis
  • Thecamonas
    • T. filosa [''Amastigomonas filosa'' {{au|(Larsen & Patterson 1990) Molina & Nerad 1991}}]
    • T. muscula [''Amastigomonas muscula'' {{au|Mylnikov 1999}}]
    • T. mutabilis [''Rhynchomonas mutabilis'' {{au|Griessmann 1913}}; ''Amastigomonas mutabilis'' {{au|(Griessmann 1913) Patterson & Zölffel 1993}}]
    • T. trahens [''Amastigomonas trahens'' {{au|(Larsen & Patterson 1990) Molina & Nerad 1991}}]

Notes and References

1. Heiss AA, Lee WJ, Ishida KI, Simpson AG. 2015. Cultivation and Characterisation of New Species of Apusomonads (the Sister Group to Opisthokonts), Including Close Relatives of Thecamonas (Chelonemonas n. gen.). Journal of Eukaryotic Microbiology. 62. 5. 637–649. 10.1111/jeu.12220. 25912654.
2. Adl SM, Bass D, Lane CE, Lukeš J, Schoch CL, Smirnov A, Agatha S, Berney C, Brown MW, Burki F, Cárdenas P, Čepička I, Chistyakova L, del Campo J, Dunthorn M, Edvardsen B, Eglit Y, Guillou L, Hampl V, Heiss AA, Hoppenrath M, James TY, Karnkowska A, Karpov S, Kim E, Kolisko M, Kudryavtsev A, ((Lahr DJG)), Lara E, Le Gall L, Lynn DH, Mann DG, Massana R, ((Mitchell EAD)), Morrow C, Park JS, Pawlowski JW, Powell MJ, Richter DJ, Rueckert S, Shadwick L, Shimano S, Spiegel FW, Torruella G, Youssef N, Zlatogursky V, Zhang Q. 2019. Revisions to the Classification, Nomenclature, and Diversity of Eukaryotes. Journal of Eukaryotic Microbiology. 66. 1 . 4–119. 10.1111/jeu.12691. 30257078 . 6492006 .