| Jurisdiction: | Apostolic Vicariate |
| Napo | |
| Latin: | Apostolicus Vicariatus Napensis |
| Country: | Ecuador |
| Area Km2: | 24,600 |
| Population: | 111,000 |
| Population As Of: | 2010 |
| Catholics: | 97,000 |
| Catholics Percent: | 87.4 |
| Parishes: | 20 |
| Denomination: | Catholic Church |
| Rite: | Roman Rite |
| Established: | 7 February 1871 (years ago) |
| Cathedral: | Catedral San José |
| Bishop: | vacant |
| Bishop Title: | Vicar Apostolic |
| Emeritus Bishops: | Adelio Pasqualotto |
| Map: | Vicariato Apostólico de Napo.png |
The Apostolic Vicariate (Vicariate Apostolic) of Napo (Latin: Apostolicus Vicariatus Napensis) is a missionary circonscription (quasi-diocese) of the Roman Catholic Church. Its cathedral see, Catedral San José, is located in the city of Tena, capital of Napo Province in Ecuador's Amazon Rainforest.
On 7 February 1871, Pope Pius IX established the Vicariate Apostolic of Napo from the Archdiocese of Quito. It remains exempt, i.e. directly subject to the Holy See, not part of any ecclesiastical province.
It has lost territory five times with the creation of the following jurisdictions within Ecuador: