| Jurisdiction: | Apostolic Vicariate |
| Puerto Ayacucho | |
| Latin: | Apostolicus Vicariatus Portus Ayacuquensis |
| Country: | Venezuela |
| Province: | Immediately exempt to the Holy See |
| Area Km2: | 183,000 |
| Population: | 219,000 |
| Population As Of: | 2010 |
| Catholics: | 167,000 |
| Catholics Percent: | 76.3 |
| Parishes: | 12 |
| Denomination: | Catholic Church |
| Sui Iuris Church: | Latin Church |
| Rite: | Roman Rite |
| Established: | 5 February 1932 (years ago) |
| Cathedral: | Catedral María Auxiliadora |
| Bishop: | Jonny Eduardo Reyes Sequera, S.D.B. |
| Bishop Title: | Vicar Apostolic |
| Map: | Vicariato apostólico de Puerto Ayacucho.svg |
The Apostolic Vicariate (or Vicariate Apostolic) of Puerto Ayacucho (Latin: Apostolicus Vicariatus Portus Ayacuquensis) is a Latin Church missionary ecclesiastical jurisdiction or apostolic vicariate of the Catholic Church in Venezuela. Its cathedral see, Catedral María Auxiliadora, is located in the town of Puerto Ayacucho, in Venezuela's Amazonas state. It is immediately exempt to the Holy See and not part of any ecclesiastical province.
On 5 February 1932 Pope Pius XI established the Prefecture Apostolic of Alto Orinoco from territory taken from the then Diocese of Santo Tomás de Guayana (which meanwhile became the Metropolitan Archdiocese of Ciudad Bolívar).
It was elevated to a Vicariate Apostolic and given its present name by Pope Pius XII on 7 May 1953.[1] [2]
So far, all incumbents have been members of the missionary Salesians (S.D.B.) congregation