Aniline Blue WS explained

Aniline Blue WS, also called aniline blue, diphenylamine blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them.^[1] It is a soluble dye used as a biological dye,^[2] in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light.^[3] It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.^[4]

Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,^[5] as well as to reveal callose structures in plant tissues.^[6]

It can also be used in other connective tissue stains, such as Mallory's stain,^[5] Gömöri trichrome stain, and Carstair's Method.^[7] It is used in differential staining.

Notes and References

1. Web site: Stainsfile - Aniline blue WS. stainsfile.info. 2012-01-14. https://web.archive.org/web/20190209123904/http://stainsfile.info/StainsFile/dyes/707.htm. 2019-02-09. dead.
2. Web site: Medical Definition of ANILINE BLUE. www.merriam-webster.com.
3. Web site: Fluorescence Microscope Images . 2012-01-14 . dead . https://web.archive.org/web/20080526080938/http://www.biologie.uni-hamburg.de/b-online/library/webb/BOT410/410Labs/Fluor-99/fluorescence_microscope_images.htm . 2008-05-26 .
4. Web site: aniline blue.
5. Web site: Stainsfile - Water blue. stainsfile.info. 2012-01-14. https://web.archive.org/web/20120207001945/http://stainsfile.info/StainsFile/dyes/42755.htm. 2012-02-07. dead.
6. Web site: https://web.archive.org/web/20050622074437/http://commonweb.unifr.ch/biol/pub/mauchgroup/staining.html. Protocols - Staining with trypan blue and aniline blue - Felix Mauch's Group. 22 June 2005.
7. Carstairs . K. C. . The Identification of platelets and platelet antigens in histological sections . The Journal of Pathology and Bacteriology . 1965 . 90 . 1 . 225–231.