| Ani Ghukasyan | |
| Birth Date: | 12 August 1990 |
| Height: | 1.53 m[1] |
| Position: | Defender |
| Currentclub: | Alashkert |
| Clubnumber: | 22 |
| Clubs1: | Artik |
| Clubs2: | Nadezhda |
| Clubs3: | Alashkert |
| Nationalteam1: | Armenia U19 |
| Nationalcaps1: | 6 |
| Nationalgoals1: | 1 |
| Nationalyears2: | 2009– |
| Nationalteam2: | Armenia |
| Nationalcaps2: | 21 |
| Nationalgoals2: | 1 |
| Nationalteam-Update: | 6 March 2020 |
Ani Ghukasyan (Armenian: Անի Ղուկասյան;[2] born 12 August 1990) is an Armenian footballer who plays as a defender for FC Alashkert and the Armenia women's national team.