| Ambrose Harmer | |
| Order: | 4th |
| Office: | Speaker of the Virginia House of Burgesses |
| Term Start: | 1646 |
| Term End: | 1646 |
| Preceded: | Edmund Scarborough |
| Succeeded: | Thomas Harwood |
| Death Date: | c. 1663 |
| Spouse: | Jane |
| Residence: | James City County, Virginia |
Ambrose Harmer (died c.1647) was a Virginia landowner and politician. An opponent of Governor Sir John Harvey, he served on the Council 1639 - 41 under his successor, Sir Francis Wyatt. He served in the House of Burgesses 1645 - 46, and was Speaker in the 1646 session.[1]