| Order: | 75th Doge of the Republic of Genoa |
| Term Start1: | November 8, 1585 |
| Term End1: | November 13, 1587 |
| Predecessor1: | Gerolamo Chiavari |
| Successor1: | Davide Vacca |
| Birth Date: | 1519 |
| Birth Place: | Genoa, Republic of Genoa |
| Death Date: | August 1601 |
| Death Place: | Genoa, Republic of Genoa |
Ambrogio Di Negro (Genoa, 1519 - Genoa, August 1601) was the 75th Doge of the Republic of Genoa.
According to the writings of the historians of the time, Ambrogio Di Negro's dogato was not easy for the continuous noble struggles, made of crime, and internal political problems, so much so that several times the Genoese government was called to revise and reform criminal justice; maneuvers that then, in fact, were minimally implemented or with not really significant results. In various memoirs, Ambrogio Di Negro's personality is described as "haughty and superb", a change of character perhaps explainable in the difficult events of his two-year term. After the end of his mandate as Doge, he preferred to retire from Genoese political life. Di Negro died in Genoa in August 1601.[1] [2]