| Alžběta Dufková | |
| Strokes: | Synchronized swimming |
| Birth Date: | 19 April 1990 |
| Birth Place: | Brno, Czech Republic |
| Height: | 171cm (67inches) |
| Weight: | 60kg (130lb) |
| Show-Medals: | yes |
Alžběta Dufková (born 19 April 1990) is a Czech synchronized swimmer who competed in the 2008 Summer Olympics, 2012 Summer Olympics and 2016 Summer Olympics. She competed with Soňa Bernadová at all three Olympics.