| Alto Biobío National Reserve | |
| Iucn Category: | IV |
| Map: | Chile |
| Relief: | 1 |
| Location: | La Araucanía Region, Chile |
| Nearest City: | Lonquimay |
| Coordinates: | -38.624°N -70.965°W |
| Coords Ref: | [1] |
| Area: | 350km2 |
| Established: | 1912 |
| Governing Body: | Corporación Nacional Forestal |
Alto Biobío National Reserve is a nature reserve located in the easternmost portion of Upper Bío-Bío River basin, in the La Araucanía Region, Chile. The vegetation is dominated by Andean steppe with scattered Araucaria trees.
Alto Biobío National Reserve is part of the Araucarias Biosphere Reserve.[2]
The reserve is crossed by the route that connects Chile and Argentina via Pino Hachado Pass.