Alternative ribosome-rescue factor B explained

Symbol:	ArfB
Uniprot:	P40711
Peptidyl-tRNA hydrolase ArfB
Organism:	E. coli

Alternative ribosome-rescue factor B (ArfB, YaeJ) also known as peptidyl-tRNA hydrolase,^[1] is a protein that plays a role in rescuing of stalled ribosomes. It works like a GGQ-release factor itself, releasing the peptide from tRNA.^[2] At the same time, it fits into the mRNA tunnel to remove the mRNA.^[3]

This gene is also found in eukaryotic organelles as MRPL58 (ICT1). The similarity is high enough to be interchangeable.^[4] Its role has expanded to that of a codon-independent release factor, although it likely still functions in ribosomal rescue.^[5]

See also

• ArfA - Alternative ribosome-rescue factor A

Notes and References

1. Web site: Peptidyl-tRNA hydrolase, ribosome rescue factor . BioCyc . SRI International .
2. Chan KH, Petrychenko V, Mueller C, Maracci C, Holtkamp W, Wilson DN, Fischer N, Rodnina MV . 6 . Mechanism of ribosome rescue by alternative ribosome-rescue factor B . Nature Communications . 11 . 1 . 4106 . August 2020 . 32796827 . 10.1038/s41467-020-17853-7 . 7427801 . 2020NatCo..11.4106C .
3. Carbone CE, Demo G, Madireddy R, Svidritskiy E, Korostelev AA . ArfB can displace mRNA to rescue stalled ribosomes . Nature Communications . 11 . 1 . 5552 . November 2020 . 33144582 . 10.1038/s41467-020-19370-z . 7641280 . 2020NatCo..11.5552C .
4. Feaga HA, Quickel MD, Hankey-Giblin PA, Keiler KC . Human Cells Require Non-stop Ribosome Rescue Activity in Mitochondria . PLOS Genetics . 12 . 3 . e1005964 . March 2016 . 27029019 . 10.1371/journal.pgen.1005964 . 4814080 . free .
5. Richter R, Rorbach J, Pajak A, Smith PM, Wessels HJ, Huynen MA, Smeitink JA, Lightowlers RN, Chrzanowska-Lightowlers ZM . 6 . A functional peptidyl-tRNA hydrolase, ICT1, has been recruited into the human mitochondrial ribosome . The EMBO Journal . 29 . 6 . 1116–1125 . March 2010 . 20186120 . 2845271. 10.1038/emboj.2010.14 .