Alpha,alpha-trehalose phosphorylase (configuration-retaining) explained
| Alpha,alpha-trehalose phosphorylase (configuration-retaining) |
| Ec Number: | 2.4.1.231 |
In enzymology, an alpha,alpha-trehalose phosphorylase (configuration-retaining) is an enzyme that catalyzes the chemical reaction
alpha,alpha-trehalose + phosphate
alpha-D-glucose + alpha-D-glucose 1-phosphate
Thus, the two substrates of this enzyme are alpha,alpha-trehalose and phosphate, whereas its two products are alpha-D-glucose and alpha-D-glucose 1-phosphate.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is alpha,alpha-trehalose:phosphate alpha-D-glucosyltransferase. This enzyme is also called trehalose phosphorylase[ambiguous].
References
- Eis C, Nidetzky B . 2002 . Substrate-binding recognition and specificity of trehalose phosphorylase from Schizophyllum commune examined in steady-state kinetic studies with deoxy and deoxyfluoro substrate analogues and inhibitors . Biochem. J. . 363 . 335–40 . 11931662 . 10.1042/0264-6021:3630335 . Pt 2 . 1222483 .
- Eis C, Watkins M, Prohaska T, Nidetzky B . 2001 . Fungal trehalose phosphorylase: kinetic mechanism, pH-dependence of the reaction and some structural properties of the enzyme from Schizophyllum commune . Biochem. J. . 356 . 757–67 . 11389683 . 10.1042/0264-6021:3560757 . Pt 3 . 1221902 .
- Nidetzky B, Eis C . 2001 . Alpha-retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune: mechanistic evidence obtained from steady-state kinetic studies with substrate analogues and inhibitors . Biochem. J. . 360 . 727–36 . 11736665 . 10.1042/0264-6021:3600727 . Pt 3 . 1222278 .
