| Alisi Tupuailei | |
| Birth Name: | Alisi Tupuailei |
| Birth Date: | 1 February 1982 |
| Birth Place: | Manunu, Samoa |
| Height: | 187cm (74inches) |
| Weight: | 116kg (256lb) |
| School: | Linwood College |
| Ru Position: | Centre, Wing |
| Ru Amateurclubs: | New Brighton |
| Ru Clubyears: | 2001–2003 2004–2010 2010–2012 2013–14 |
| Ru Proclubs: | Canterbury Honda Heat Canon Eagles Navy SC |
| Ru Clubcaps: | 12 |
| Ru Clubpoints: | (80) |
| Ru Nationalyears: | 2001 2009-11 |
| Ru Nationalteam: | New Zealand U21 Japan |
| Ru Nationalcaps: | 20 |
| Ru Nationalpoints: | (100) |
| Repsevensyears1: | 2009-12 |
| Repsevensteam1: | Japan |
Alisi Tupuailei (born 1 February 1982) is a former professional rugby union player who played as a centre. Born in Samoa, he played internationally for the Japan national team.
Tupuailei played for Canterbury in the provincial games. He has represented Japan in both sevens and fifteens. He made his Rugby World Cup debut in 2011. He was part of the Japanese squad to the 2012 Hong Kong Sevens.