Aliphatic (R)-hydroxynitrile lyase explained

Aliphatic (R)-hydroxynitrile lyase
Ec Number:	4.1.2.46

Aliphatic (R)-hydroxynitrile lyase ((R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase, LuHNL) is an enzyme with systematic name (2R)-2-hydroxy-2-methylbutanenitrile butan-2-one-lyase (cyanide forming).^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction:

(2R)-2-hydroxy-2-methylbutanenitrile

\rightleftharpoons cyanide + butan-2-one

The enzyme contains Zn²⁺.

Notes and References

1. Expression of the Zn²⁺-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris - utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis . Trummler, K. . Roos, J. . Schwaneberg, U. . Effenberger, F. . Förster, S. . Pfizenmaier, K. . Wajant, H. . Plant Sci. . 1998 . 139 . 19–27 . 10.1016/s0168-9452(98)00173-3.
2. Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases . Trummler, K. . Wajant, H. . J. Biol. Chem. . 1997 . 272 . 4770–4774 . 9030531 . 8 . 10.1074/jbc.272.8.4770. free .
3. Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range . Albrecht J, Jansen I, Kula MR . Biotechnol. Appl. Biochem. . 1993 . 17 . 2 . 191–203 . 8387315.
4. Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum . Xu, L.-L. . Singh, B.K. . Conn, E.E. . Arch. Biochem. Biophys. . 1988 . 263 . 2 . 256–263 . 3377504 . 10.1016/0003-9861(88)90634-0.
5. Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst . Cabirol, F.L. . Tan, P.L. . Tay, B. . Cheng, S. . Hanefeld, U. . Roger A. Sheldon . Sheldon, R.A. . Adv. Synth. Catal. . 2008 . 350 . 14–15 . 2329–2338 . 10.1002/adsc.200800309.
6. Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax) . Breithaupt, H. . Pohl, M. . Bönigk, W. . Heim, P. . Schimz, K.-L. . Kula, M.-R. . J. Mol. Catal. B . 1999 . 6 . 3 . 315–332 . 10.1016/S1381-1177(98)00109-X .