| Honorific-Prefix: | Honourable |
| Ali Khamis Seif | |
| Honorific-Suffix: | MP |
| Office: | Member of Parliament for Mkoani |
| Term Start: | December 2005 |
| Predecessor: | Issa Mohamed Salim |
| Birth Date: | 8 April 1954 |
| Birth Place: | Sultanate of Zanzibar |
| Nationality: | Tanzanian |
| Party: | CUF |
| Alma Mater: | Nkrumah Teachers' Training College |
Ali Khamis Seif (born 8 April 1954) is a Tanzanian CUF politician and Member of Parliament for Mkoani constituency since 2005.[1]