| Honorific-Prefix: | Most Reverend |
| Alfonso de la Mota y Escobar | |
| Bishop of Tlaxcala (Puebla de los Angeles) | |
| Church: | Catholic Church |
| Diocese: | Diocese of Tlaxcala (Puebla de los Angeles) |
| Term: | 1607–1625 |
| Predecessor: | Diego de Romano y Govea |
| Successor: | Gutiérrez Bernardo de Quirós |
| Consecration: | June 1599 |
| Birth Date: | 18 May 1546 |
| Birth Place: | México |
| Death Date: | 16 March 1625 |
| Death Place: | Tlaxcala, México |
| Previous Post: | Bishop of Santiago de Cuba (1580–1597) |
Alfonso de la Mota y Escobar (18 May 1546 – 16 March 1625) was a Roman Catholic prelate who served as Bishop of Tlaxcala (Puebla de los Angeles) (1607–1625),[1] Bishop of Guadalajara (1598–1607),[2] and Bishop Elect of Nicaragua (1594–1595).
Alfonso de la Mota y Escobar was born in México on 18 May 1546.[3] [4] On 31 March 1594, he was appointed during the papacy of Pope Clement VIII as Bishop of Nicaragua but resigned in 1595. On 11 March 1598, he was appointed during the papacy of Pope Clement VIII as Bishop of Guadalajara.In June 1599, he was consecrated bishop On 11 March 1598, he was appointed during the papacy of Pope Clement VIII as Bishop of Tlaxcala (Puebla de los Angeles). He served as Bishop of Tlaxcala (Puebla de los Angeles) until his death on 16 March 1625.
While bishop, he was the principal consecrator of Juan de Cervantes (bishop), Bishop of Antequera, Oaxaca (1609), and Juan de Zapata y Sandoval, Bishop of Chiapas (1613).