Alanine dehydrogenase explained
| alanine dehydrogenase |
| Ec Number: | 1.4.1.1 |
| Cas Number: | 9029-06-5 |
| Go Code: | 0000286 |
Alanine dehydrogenase is an enzyme that catalyzes the chemical reaction
L-alanine + H2O + NAD+
pyruvate + NH
3 + NADH + H
+The 2 substrates of this enzyme are L-alanine, water, and nicotinamide adenine dinucleotide+ because water is 55M and does not change, whereas its 4 products are pyruvate, ammonia, NADH, and hydrogen ion.
This enzyme participates in taurine and hypotaurine metabolism and reductive carboxylate cycle (fixation).
Nomenclature
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is L-alanine:NAD+ oxidoreductase (deaminating). Other names in common use include AlaDH, L-alanine dehydrogenase, NAD+-linked alanine dehydrogenase, alpha-alanine dehydrogenase, NAD+-dependent alanine dehydrogenase, alanine oxidoreductase, and NADH-dependent alanine dehydrogenase. T
Structure
Alanine dehydrogenase contains both a N-terminus and C-terminus domains.[1]
Further reading
- O'Connor RJ, Halvorson H . The substrate specificity of L-alanine dehydrogenase . Biochimica et Biophysica Acta . 48 . 1 . 47–55 . March 1961 . 13730044 . 10.1016/0006-3002(61)90513-3 .
- Pierard A . Wiame JM . 1960 . Proprietes de la L(+)-alanine-deshydrogenase . Biochim. Biophys. Acta . 37 . 3 . 490 - 502 . 10.1016/0006-3002(60)90506-0 . 14432812 .
- Yoshida A, Freese E . Enzymic properties of alanine dehydrogenase of Bacillus subtilis . Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation . 96 . 248–62 . February 1965 . 2 . 14298830 . 10.1016/0926-6593(65)90009-3 .
- Tripathi SM, Ramachandran R . Crystal structures of the Mycobacterium tuberculosis secretory antigen alanine dehydrogenase (Rv2780) in apo and ternary complex forms captures "open" and "closed" enzyme conformations . Proteins . 72 . 3 . 1089–95 . August 2008 . 18491387 . 10.1002/prot.22101 . 23999004 .
Notes and References
- Tripathi SM, Ramachandran R . Crystal structures of the Mycobacterium tuberculosis secretory antigen alanine dehydrogenase (Rv2780) in apo and ternary complex forms captures "open" and "closed" enzyme conformations . Proteins . 72 . 3 . 1089–95 . 2008 . 18491387 . 10.1002/prot.22101 . 23999004 .
