| Railroad Name: | Alabama and Florida Railroad |
| Image Alt: | Picture showing red locomotive Alabama & Florida 6011 |
| Marks: | AFLR |
| Successor Line: | Alabama and Florida Railway |
The Alabama and Florida Railroad was a short-line railroad which operated in Alabama and Florida. The company operated a former Louisville and Nashville Railroad (L&N) line between Georgiana, Alabama and Geneva, Alabama. Pioneer Railcorp acquired the line in 1992 and created a new company, the Alabama and Florida Railway, to operate it.
See main article: Alabama and Florida Railroad (1898–1900). The Alabama and Florida (A&F) owned two routes: Georgiana, Alabama–Geneva, Alabama and Crestview, Florida–Florala, Alabama. The Georgiana line was begun in 1898 by the Alabama and Florida Railroad and completed by the Louisville and Nashville Railroad in 1902. The Crestview line opened in 1894 under the Yellow River Railroad, which was acquired by the L&N in 1906. Both lines passed to the Seaboard System Railroad, the L&N's successor, in 1982.
Gulf and Ohio Railways purchased both lines in 1986 and created a new subsidiary, the Alabama and Florida Railroad, to operate them. The railroad began operating on July 28, 1986. The company abandoned the former Yellow River Railroad in December 1987. The A&F also leased a 2.9miles line from the Andalusia and Conecuh Railroad in the vicinity of Andalusia, Alabama.
The Gulf and Ohio sold the railroad to A&F Inc. on August 12, 1990. Pioneer Railcorp acquired the assets of the company on November 23, 1992, and organized a new railroad, the Alabama and Florida Railway, to operate the lines.