| State: | AL |
| Type: | AL |
| Route: | 27 |
| Map Custom: | yes |
| Length Mi: | 77.122 |
| Length Ref: | [1] |
| Direction A: | South |
| Direction B: | North |
| Terminus A: | at Florida state line southwest of Geneva, AL |
| Junction: | in Geneva in Enterprise in Ozark near Abbeville |
| Terminus B: | in Abbeville |
| Counties: | Geneva, Coffee, Dale, Henry |
| Previous Type: | AL |
| Previous Route: | 26 |
| Next Type: | AL |
| Next Route: | 28 |
State Route 27 (SR 27) is a 77.122adj=midNaNadj=mid state highway in the southeastern part of the U.S. state of Alabama. The southern terminus of the highway is at the Florida state line, where the route serves as a continuation of County Road 185 (CR 185). The northern terminus of the highway is at the intersection with SR 10 at Abbeville.
Entering Alabama in eastern Geneva County, SR 27 is primarily a two-lane highway. It serves as a connector from the Gulf coast beaches along the Florida Panhandle to major highways such as US 84 and US 231. Between the cities of Geneva and Enterprise, the highway's orientation is generally north-to-south. Upon leaving Enterprise, the highway assumes a northeastward trajectory as it leads into Dale County. East of Ozark, the highway, while still signed as “north” and “south”, assumes an east–west orientation until its terminus at Abbeville, at SR 10.