Acireductone synthase explained
| Acireductone synthase |
| Ec Number: | 3.1.3.77 |
Acireductone synthase (EC number 3.1.3.77, E1, E-1 enolase-phosphatase) is an enzyme with systematic name 5-(methylsulfanyl)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing).[1] [2] [3] It catalyses the following reaction:
5-(methylsulfanyl)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate (overall reaction)
(1a) 5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate (probably spontaneous)
(1b) 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate
Notes and References
- Myers RW, Wray JW, Fish S, Abeles RH . Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae . The Journal of Biological Chemistry . 268 . 33 . 24785–91 . November 1993 . 8227039 .
- Wray JW, Abeles RH . The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases . The Journal of Biological Chemistry . 270 . 7 . 3147–53 . February 1995 . 7852397 . 10.1074/jbc.270.7.3147 . free .
- Wang H, Pang H, Bartlam M, Rao Z . Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity . Journal of Molecular Biology . 348 . 4 . 917–26 . May 2005 . 15843022 . 10.1016/j.jmb.2005.01.072 .
