A-1,6-mannosyl-glycoprotein 2-b-N-acetylglucosaminyltransferase explained

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Ec Number:	2.4.1.143
Cas Number:	105913-04-0

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase II, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II, acetylglucosaminyltransferase II, uridine diphosphoacetylglucosamine-mannoside alpha1->6-acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-D-mannoside beta1-2-acetylglucosaminyltransferase, UDP-GlcNAc:mannoside alpha1-6 acetylglucosaminyltransferase, alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, GnTII) is an enzyme with systematic name UDP-N-acetyl-D-glucosamine:6-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase.^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R

\rightleftharpoons UDP + 6-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R

R represents the remainder of the N-linked oligosaccharide in the glycoprotein acceptor.

Notes and References

1. Bendiak B, Schachter H . Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver . The Journal of Biological Chemistry . 262 . 12 . 5775–83 . April 1987 . 2952644 .
2. Harpaz N, Schachter H . Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity . The Journal of Biological Chemistry . 255 . 10 . 4885–93 . May 1980 . 6445358 .
3. Mendicino J, Chandrasekaran EV, Anumula KR, Davila M . Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa . Biochemistry . 20 . 4 . 967–76 . February 1981 . 6452163 . 10.1021/bi00507a050 .
4. Oppenheimer CL, Eckhardt AE, Hill RL . The nonidentity of porcine N-acetylglucosaminyltransferases I and II . The Journal of Biological Chemistry . 256 . 22 . 11477–82 . November 1981 . 6457827 .
5. Book: Schachter H, Narasimhan S, Gleeson P, Vella G . Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type . Methods in Enzymology . 98 . 98–134 . 1983 . 6366476 . 10.1016/0076-6879(83)98143-0 .