| Official Name: | Acassuso |
| Pushpin Map: | Argentina Greater Buenos Aires |
| Pushpin Label Position: | bottom |
| Pushpin Map Caption: | Location in Greater Buenos Aires |
| Pushpin Mapsize: | 240 |
| Subdivision Type: | Country |
| Subdivision Type1: | Province |
| Subdivision Name1: | Buenos Aires |
| Subdivision Type2: | Partido |
| Subdivision Name2: | San Isidro |
| Population Total: | 12842 |
| Coordinates: | -34.4667°N -89°W |
| Elevation M: | 18 |
| Postal Code Type: | CPA Base |
| Postal Code: | B 1640 |
| Area Code: | +54 11 |
Acassuso is a locality in the San Isidro "partido" of Buenos Aires Province. It is about 16 kilometres north of the city of Buenos Aires, between Olivos and San Isidro. It borders on the River Plate where an approximately 10 hectare municipal nature reserve "Refugio Natural Educativo de la Ribera Norte" exists since 1994. It is served by two commuter train services, the Tren de la Costa tourist line and the Mitre line.