ATP6V0E1 explained

V-type proton ATPase subunit e 1 is an enzyme that in humans is encoded by the ATP6V0E1 gene.^{[1] [2] [3]}

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dogs, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3.

Further reading

• Finbow ME, Harrison MA . The vacuolar H+-ATPase: a universal proton pump of eukaryotes . Biochemical Journal . 324 . 3. 697–712 . 1997 . 9210392 . 10.1042/bj3240697. 1218484 .
• Stevens TH, Forgac M . Structure, function and regulation of the vacuolar (H+)-ATPase . Annual Review of Cell and Developmental Biology . 13 . 779–808 . 1998 . 9442887 . 10.1146/annurev.cellbio.13.1.779 .
• Nelson N, Harvey WR . Vacuolar and plasma membrane proton-adenosinetriphosphatases . Physiological Reviews . 79 . 2 . 361–85 . 1999 . 10221984 . 10.1152/physrev.1999.79.2.361. 1477911 .
• Forgac M . Structure and properties of the vacuolar (H+)-ATPases . Journal of Biological Chemistry . 274 . 19 . 12951–12954 . 1999 . 10224039 . 10.1074/jbc.274.19.12951 . free .
• Kane PM . Introduction: V-ATPases 1992-1998 . Journal of Bioenergetics and Biomembranes . 31 . 1 . 3–5 . 1999 . 10340843 . 10.1023/A:1001884227654 .
• Wieczorek H . Animal plasma membrane energization by proton-motive V-ATPases . BioEssays . 21 . 8 . 637–648 . 1999 . 10440860 . 10.1002/(SICI)1521-1878(199908)21:8<637::AID-BIES3>3.0.CO;2-W . Brown D . Grinstein S . Ehrenfeld . Jordi . Harvey . William R. . 23505139 .
• Nishi T, Forgac M . The vacuolar (H+)-ATPases--nature's most versatile proton pumps . Nature Reviews Molecular Cell Biology . 3 . 2 . 94–103 . 2002 . 11836511 . 10.1038/nrm729 . 21122465 .
• Kawasaki-Nishi S, Nishi T, Forgac M . Proton translocation driven by ATP hydrolysis in V-ATPases . FEBS Letters . 545 . 1 . 76–85 . 2003 . 12788495 . 10.1016/S0014-5793(03)00396-X . 10507213 .
• Morel N . Neurotransmitter release: the dark side of the vacuolar-H+ATPase . Biology of the Cell . 95 . 7 . 453–457 . 2004 . 14597263 . 10.1016/S0248-4900(03)00075-3 . 17519696 . free .
• Lu M . The amino-terminal domain of the E subunit of vacuolar H(+)-ATPase (V-ATPase) interacts with the H subunit and is required for V-ATPase function . Journal of Biological Chemistry . 277 . 41 . 38409–38415 . 2002 . 12163484 . 10.1074/jbc.M203521200 . Vergara S . Zhang L . Holliday . LS . Aris . J . Gluck . SL . free.
• Strausberg RL . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proceedings of the National Academy of Sciences of the United States of America . 99 . 26 . 16899–16903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . Feingold EA . Grouse LH . Derge . JG . Klausner . RD . Collins . FS . Wagner . L . Shenmen . CM . Schuler . GD . 2002PNAS...9916899M . free .

Notes and References

1. Ludwig J, Kerscher S, Brandt U, Pfeiffer K, Getlawi F, Apps DK, Schagger H . Identification and characterization of a novel 9.2-kDa membrane sector-associated protein of vacuolar proton-ATPase from chromaffin granules . Journal of Biological Chemistry . 273 . 18 . 10939–10947 . June 1998 . 9556572 . 10.1074/jbc.273.18.10939 . free .
2. Sambade M, Kane PM . The yeast vacuolar proton-translocating ATPase contains a subunit homologous to the Manduca sexta and bovine e subunits that is essential for function . Journal of Biological Chemistry . 279 . 17 . 17361–17365 . April 2004 . 14970230 . 10.1074/jbc.M314104200 . free .
3. Web site: Entrez Gene: ATP6V0E1 ATPase, H+ transporting, lysosomal 9kDa, V0 subunit e1.