ATP2B2 explained

Plasma membrane calcium-transporting ATPase 2 is an enzyme that in humans is encoded by the ATP2B2 gene.^{[1] [2]}

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified.

Further reading

• Møller JV, Juul B, le Maire M . Structural organization, ion transport, and energy transduction of P-type ATPases. . Biochim. Biophys. Acta . 1286 . 1 . 1–51 . 1996 . 8634322 . 10.1016/0304-4157(95)00017-8.
• Strehler EE, Zacharias DA . Role of alternative splicing in generating isoform diversity among plasma membrane calcium pumps. . Physiol. Rev. . 81 . 1 . 21–50 . 2001 . 11152753 . 10.1152/physrev.2001.81.1.21. 9062253 .
• Strehler EE, Treiman M . Calcium pumps of plasma membrane and cell interior. . Curr. Mol. Med. . 4 . 3 . 323–35 . 2004 . 15101689 . 10.2174/1566524043360735 .
• Brandt P, Ibrahim E, Bruns GA, Neve RL . Determination of the nucleotide sequence and chromosomal localization of the ATP2B2 gene encoding human Ca(2+)-pumping ATPase isoform PMCA2. . Genomics . 14 . 2 . 484–7 . 1992 . 1427863 . 10.1016/S0888-7543(05)80246-0 .
• Wang KK, Wright LC, Machan CL . Protein kinase C phosphorylates the carboxyl terminus of the plasma membrane Ca(2+)-ATPase from human erythrocytes. . J. Biol. Chem. . 266 . 14 . 9078–85 . 1991 . 10.1016/S0021-9258(18)31554-0 . 1827443 . etal. free .
• Stauffer TP, Hilfiker H, Carafoli E, Strehler EE . Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes. . J. Biol. Chem. . 269 . 50 . 32022 . 1995 . 10.1016/S0021-9258(18)31797-6 . 7989379 . free .
• Kuzmin I, Stackhouse T, Latif F . One-megabase yeast artificial chromosome and 400-kilobase cosmid-phage contigs containing the von Hippel-Lindau tumor suppressor and Ca(2+)-transporting adenosine triphosphatase isoform 2 genes. . Cancer Res. . 54 . 9 . 2486–91 . 1994 . 8162598 . etal.
• Wang MG, Yi H, Hilfiker H . Localization of two genes encoding plasma membrane Ca2+ ATPases isoforms 2 (ATP2B2) and 3 (ATP2B3) to human chromosomes 3p26→p25 and Xq28, respectively. . Cytogenet. Cell Genet. . 67 . 1 . 41–5 . 1994 . 8187550 . 10.1159/000133794 . etal.
• Stauffer TP, Hilfiker H, Carafoli E, Strehler EE . Quantitative analysis of alternative splicing options of human plasma membrane calcium pump genes. . J. Biol. Chem. . 268 . 34 . 25993–6003 . 1994 . 10.1016/S0021-9258(19)74484-6 . 8245032 . free .
• Latif F, Duh FM, Gnarra J . von Hippel-Lindau syndrome: cloning and identification of the plasma membrane Ca(++)-transporting ATPase isoform 2 gene that resides in the von Hippel-Lindau gene region. . Cancer Res. . 53 . 4 . 861–7 . 1993 . 8428366 . etal.
• Kozel PJ, Friedman RA, Erway LC . Balance and hearing deficits in mice with a null mutation in the gene encoding plasma membrane Ca2+-ATPase isoform 2. . J. Biol. Chem. . 273 . 30 . 18693–6 . 1998 . 9668038 . 10.1074/jbc.273.30.18693 . etal. free .
• Street VA, McKee-Johnson JW, Fonseca RC . Mutations in a plasma membrane Ca2+-ATPase gene cause deafness in deafwaddler mice. . Nat. Genet. . 19 . 4 . 390–4 . 1998 . 9697703 . 10.1038/1284 . 43382998 . etal.
• Usachev YM, Toutenhoofd SL, Goellner GM . Differentiation induces up-regulation of plasma membrane Ca(2+)-ATPase and concomitant increase in Ca(2+) efflux in human neuroblastoma cell line IMR-32. . J. Neurochem. . 76 . 6 . 1756–65 . 2001 . 11259493 . 10.1046/j.1471-4159.2001.00169.x . 5721693 . etal. free .
• DeMarco SJ, Strehler EE . Plasma membrane Ca2+-atpase isoforms 2b and 4b interact promiscuously and selectively with members of the membrane-associated guanylate kinase family of PDZ (PSD95/Dlg/ZO-1) domain-containing proteins. . J. Biol. Chem. . 276 . 24 . 21594–600 . 2001 . 11274188 . 10.1074/jbc.M101448200 . free .
• DeMarco SJ, Chicka MC, Strehler EE . Plasma membrane Ca2+ ATPase isoform 2b interacts preferentially with Na+/H+ exchanger regulatory factor 2 in apical plasma membranes. . J. Biol. Chem. . 277 . 12 . 10506–11 . 2002 . 11786550 . 10.1074/jbc.M111616200 . free .
• Wistow G, Bernstein SL, Wyatt MK . Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants. . Mol. Vis. . 8 . 205–20 . 2002 . 12107410 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .

Notes and References

1. Heim R, Hug M, Iwata T, Strehler EE, Carafoli E . Microdiversity of human-plasma-membrane calcium-pump isoform 2 generated by alternative RNA splicing in the N-terminal coding region . Eur J Biochem . 205 . 1 . 333–40 . May 1992 . 1313367 . 10.1111/j.1432-1033.1992.tb16784.x . free .
2. Web site: Entrez Gene: ATP2B2 ATPase, Ca++ transporting, plasma membrane 2.