ASPH explained

Aspartyl/asparaginyl beta-hydroxylase (HAAH) is an enzyme that in humans is encoded by the ASPH gene.[1] [2] [3] ASPH is an alpha-ketoglutarate-dependent hydroxylase, a superfamily non-haem iron-containing proteins.

Function

This gene is thought to play an important role in calcium homeostasis. Alternative splicing of this gene results in five transcript variants which vary in protein translation, the coding of catalytic domains, and tissue expression. Variation among these transcripts impacts their functions which involve roles in the calcium storage and release process in the endoplasmic and sarcoplasmic reticulum as well as hydroxylation of aspartic acid and asparagine in epidermal growth factor-like domains of various proteins.

Clinical significance

As early as 1996, the over-expression of HAAH was recognized as an indicator of carcinoma in humans. Further research has correlated elevated HAAH levels (variously in affected tissue or blood serum) with hepatocellular (liver) carcinoma[4] [5] adenocarcinoma (pancreatic cancer),[6] colorectal cancer,[7] prostate cancer. and lung cancer.[8] The pancreatic study showed elevated HAAH only in diseased tissue, but not in adjacent normal and inflamed tissue.

Mutations in ASPH cause Traboulsi syndrome.[9]

Further reading

Notes and References

  1. Korioth F, Gieffers C, Frey J . Cloning and characterization of the human gene encoding aspartyl beta-hydroxylase . Gene . 150 . 2 . 395–9 . December 1994 . 7821814 . 10.1016/0378-1119(94)90460-X .
  2. Lim KY, Hong CS, Kim DH . cDNA cloning and characterization of human cardiac junctin . Gene . 255 . 1 . 35–42 . September 2000 . 10974562 . 10.1016/S0378-1119(00)00299-7 .
  3. Web site: Entrez Gene: ASPH aspartate beta-hydroxylase.
  4. Ince N, de la Monte SM, Wands JR . Overexpression of human aspartyl (asparaginyl) beta-hydroxylase is associated with malignant transformation . Cancer Research . 60 . 5 . 1261–6 . March 2000 . 10728685 .
  5. Xue T, Xue XP, Huang QS, Wei L, Sun K, Xue T . Monoclonal antibodies against human aspartyl (asparaginyl) beta-hydroxylase developed by DNA immunization . Hybridoma . 28 . 4 . 251–7 . August 2009 . 19663697 . 10.1089/hyb.2009.0017 .
  6. Palumbo KS, Wands JR, Safran H, King T, Carlson RI, de la Monte SM . Human aspartyl (asparaginyl) beta-hydroxylase monoclonal antibodies: potential biomarkers for pancreatic carcinoma . Pancreas . 25 . 1 . 39–44 . July 2002 . 12131769 . 10.1097/00006676-200207000-00010 . 2098747 .
  7. Web site: CC Detect - Serum-Based Diagnostic Test For Colon Cancer Available . 2011-11-29 . 2021-02-08 . https://web.archive.org/web/20210208135602/https://www.emaxhealth.com/100/18177.html . dead .
  8. Hampton T . New screening techniques show potential for early detection of lung cancer . JAMA . 298 . 17 . 1997 . November 2007 . 17986689 . 10.1001/jama.298.17.1997 .
  9. Patel N, Khan AO, Mansour A, Mohamed JY, Al-Assiri A, Haddad R, Jia X, Xiong Y, Mégarbané A, Traboulsi EI, Alkuraya FS . 6 . Mutations in ASPH cause facial dysmorphism, lens dislocation, anterior-segment abnormalities, and spontaneous filtering blebs, or Traboulsi syndrome . American Journal of Human Genetics . 94 . 5 . 755–9 . May 2014 . 24768550 . 4067561 . 10.1016/j.ajhg.2014.04.002 .