ARHGAP1 explained

Rho GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARHGAP1 gene.^{[1] [2]}

Interactions

ARHGAP1 has been shown to interact with:

• BNIP2^[3]
• CDC42,^{[4] [5] [6] [7]} and
• RHOA.^{[6] [7] [8] [9]}

Further reading

• Diekmann D, Brill S, Garrett MD, Totty N, Hsuan J, Monfries C, Hall C, Lim L, Hall A . Bcr encodes a GTPase-activating protein for p21rac . Nature . 351 . 6325 . 400–2 . 1991 . 1903516 . 10.1038/351400a0 . 1991Natur.351..400D . 4233469 .
• Garrett MD, Major GN, Totty N, Hall A . Purification and N-terminal sequence of the p21rho GTPase-activating protein, rho GAP . Biochem. J. . 276 (Pt 3) . 3 . 833–6 . 1991 . 1905930 . 1151079 . 10.1042/bj2760833.
• Barfod ET, Zheng Y, Kuang WJ, Hart MJ, Evans T, Cerione RA, Ashkenazi A . Cloning and expression of a human CDC42 GTPase-activating protein reveals a functional SH3-binding domain . J. Biol. Chem. . 268 . 35 . 26059–62 . 1993 . 10.1016/S0021-9258(19)74277-X . 8253717 . free .
• Aspenström P, Lindberg U, Hall A . Two GTPases, Cdc42 and Rac, bind directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich syndrome . Curr. Biol. . 6 . 1 . 70–5 . 1996 . 8805223 . 10.1016/S0960-9822(02)00423-2 . free . 1996CBio....6...70A .
• Barrett T, Xiao B, Dodson EJ, Dodson G, Ludbrook SB, Nurmahomed K, Gamblin SJ, Musacchio A, Smerdon SJ, Eccleston JF . The structure of the GTPase-activating domain from p50rhoGAP . Nature . 385 . 6615 . 458–61 . 1997 . 9009196 . 10.1038/385458a0 . 1997Natur.385..458B . 4235645 .
• Hu KQ, Settleman J . Tandem SH2 binding sites mediate the RasGAP-RhoGAP interaction: a conformational mechanism for SH3 domain regulation . EMBO J. . 16 . 3 . 473–83 . 1997 . 9034330 . 1169651 . 10.1093/emboj/16.3.473 .
• Rittinger K, Walker PA, Eccleston JF, Nurmahomed K, Owen D, Laue E, Gamblin SJ, Smerdon SJ . Crystal structure of a small G protein in complex with the GTPase-activating protein rhoGAP . Nature . 388 . 6643 . 693–7 . 1997 . 9262406 . 10.1038/41805 . 4415826 . free .
• Zhang B, Wang ZX, Zheng Y . Characterization of the interactions between the small GTPase Cdc42 and its GTPase-activating proteins and putative effectors. Comparison of kinetic properties of Cdc42 binding to the Cdc42-interactive domains . J. Biol. Chem. . 272 . 35 . 21999–2007 . 1997 . 9268338 . 10.1074/jbc.272.35.21999 . free .
• Li R, Zhang B, Zheng Y . Structural determinants required for the interaction between Rho GTPase and the GTPase-activating domain of p190 . J. Biol. Chem. . 272 . 52 . 32830–5 . 1997 . 9407060 . 10.1074/jbc.272.52.32830 . free .
• Zhang B, Zheng Y . Regulation of RhoA GTP hydrolysis by the GTPase-activating proteins p190, p50RhoGAP, Bcr, and 3BP-1 . Biochemistry . 37 . 15 . 5249–57 . 1998 . 9548756 . 10.1021/bi9718447 .
• Low BC, Lim YP, Lim J, Wong ES, Guy GR . Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42 . J. Biol. Chem. . 274 . 46 . 33123–30 . 1999 . 10551883 . 10.1074/jbc.274.46.33123 . free .
• Graham DL, Eccleston JF, Chung CW, Lowe PN . Magnesium fluoride-dependent binding of small G proteins to their GTPase-activating proteins . Biochemistry . 38 . 45 . 14981–7 . 1999 . 10555980 . 10.1021/bi991358e .
• Low BC, Seow KT, Guy GR . Evidence for a novel Cdc42GAP domain at the carboxyl terminus of BNIP-2 . J. Biol. Chem. . 275 . 19 . 14415–22 . 2000 . 10799524 . 10.1074/jbc.275.19.14415 . free .
• Low BC, Seow KT, Guy GR . The BNIP-2 and Cdc42GAP homology domain of BNIP-2 mediates its homophilic association and heterophilic interaction with Cdc42GAP . J. Biol. Chem. . 275 . 48 . 37742–51 . 2000 . 10954711 . 10.1074/jbc.M004897200 . free .
• Zhou YT, Soh UJ, Shang X, Guy GR, Low BC . The BNIP-2 and Cdc42GAP homology/Sec14p-like domain of BNIP-Salpha is a novel apoptosis-inducing sequence . J. Biol. Chem. . 277 . 9 . 7483–92 . 2002 . 11741952 . 10.1074/jbc.M109459200 . free .
• Fidyk NJ, Cerione RA . Understanding the catalytic mechanism of GTPase-activating proteins: demonstration of the importance of switch domain stabilization in the stimulation of GTP hydrolysis . Biochemistry . 41 . 52 . 15644–53 . 2002 . 12501193 . 10.1021/bi026413p .
• Qin W, Hu J, Guo M, Xu J, Li J, Yao G, Zhou X, Jiang H, Zhang P, Shen L, Wan D, Gu J . BNIPL-2, a novel homologue of BNIP-2, interacts with Bcl-2 and Cdc42GAP in apoptosis . Biochem. Biophys. Res. Commun. . 308 . 2 . 379–85 . 2003 . 12901880 . 10.1016/S0006-291X(03)01387-1 .
• Shang X, Zhou YT, Low BC . Concerted regulation of cell dynamics by BNIP-2 and Cdc42GAP homology/Sec14p-like, proline-rich, and GTPase-activating protein domains of a novel Rho GTPase-activating protein, BPGAP1 . J. Biol. Chem. . 278 . 46 . 45903–14 . 2003 . 12944407 . 10.1074/jbc.M304514200 . free .

Notes and References

1. Lancaster CA, Taylor-Harris PM, Self AJ, Brill S, van Erp HE, Hall A . Characterization of rhoGAP. A GTPase-activating protein for rho-related small GTPases . J. Biol. Chem. . 269 . 2 . 1137–42 . February 1994 . 10.1016/S0021-9258(17)42232-0 . 8288572 . free .
2. Web site: Entrez Gene: ARHGAP1 Rho GTPase activating protein 1.
3. Low BC, Seow KT, Guy GR . The BNIP-2 and Cdc42GAP homology domain of BNIP-2 mediates its homophilic association and heterophilic interaction with Cdc42GAP . J. Biol. Chem. . 275 . 48 . 37742–51 . December 2000 . 10954711 . 10.1074/jbc.M004897200 . free .
4. Low BC, Lim YP, Lim J, Wong ES, Guy GR . Tyrosine phosphorylation of the Bcl-2-associated protein BNIP-2 by fibroblast growth factor receptor-1 prevents its binding to Cdc42GAP and Cdc42 . J. Biol. Chem. . 274 . 46 . 33123–30 . November 1999 . 10551883 . 10.1074/jbc.274.46.33123 . free .
5. ((Nagata Ki)), Puls A, Futter C, Aspenstrom P, Schaefer E, Nakata T, Hirokawa N, Hall A . The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3 . EMBO J. . 17 . 1 . 149–58 . January 1998 . 9427749 . 1170366 . 10.1093/emboj/17.1.149 .
6. Li R, Zhang B, Zheng Y . Structural determinants required for the interaction between Rho GTPase and the GTPase-activating domain of p190 . J. Biol. Chem. . 272 . 52 . 32830–5 . December 1997 . 9407060 . 10.1074/jbc.272.52.32830 . free .
7. Zhang B, Chernoff J, Zheng Y . Interaction of Rac1 with GTPase-activating proteins and putative effectors. A comparison with Cdc42 and RhoA . J. Biol. Chem. . 273 . 15 . 8776–82 . April 1998 . 9535855 . 10.1074/jbc.273.15.8776 . free .
8. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M . Towards a proteome-scale map of the human protein-protein interaction network . Nature . 437 . 7062 . 1173–8 . October 2005 . 16189514 . 10.1038/nature04209 . 2005Natur.437.1173R . 4427026 .
9. Zhang B, Zheng Y . Regulation of RhoA GTP hydrolysis by the GTPase-activating proteins p190, p50RhoGAP, Bcr, and 3BP-1 . Biochemistry . 37 . 15 . 5249–57 . April 1998 . 9548756 . 10.1021/bi9718447 .