Annexin A4 Explained

Annexin A4 is a protein that in humans is encoded by the ANXA4 gene.^{[1] [2]}

Function

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.^[2]

Further reading

• Römisch J, Heimburger N . Purification and characterization of six annexins from human placenta . Biological Chemistry Hoppe-Seyler . 371 . 5 . 383–8 . May 1990 . 2143074 . 10.1515/bchm3.1990.371.1.383 .
• Römisch J, Grote M, Weithmann KU, Heimburger N, Amann E . Annexin proteins PP4 and PP4-X. Comparative characterization of biological activities of placental and recombinant proteins . The Biochemical Journal . 272 . 1 . 223–9 . Nov 1990 . 2148260 . 1149680 . 10.1042/bj2720223.
• Freemont PS, Driessen HP, Verbi W, Crumpton MJ . Crystallization and preliminary X-ray crystallographic studies of human placental annexin IV . Journal of Molecular Biology . 216 . 2 . 219–21 . Nov 1990 . 2254922 . 10.1016/S0022-2836(05)80310-9 .
• Hauptmann R, Maurer-Fogy I, Krystek E, Bodo G, Andree H, Reutelingsperger CP . Vascular anticoagulant beta: a novel human Ca2+/phospholipid binding protein that inhibits coagulation and phospholipase A2 activity. Its molecular cloning, expression and comparison with VAC-alpha . European Journal of Biochemistry . 185 . 1 . 63–71 . Oct 1989 . 2530088 . 10.1111/j.1432-1033.1989.tb15082.x . free .
• Grundmann U, Amann E, Abel KJ, Küpper HA . Isolation and expression of cDNA coding for a new member of the phospholipase A2 inhibitor family . Behring Institute Mitteilungen . 82 . 59–67 . Apr 1988 . 2970257 .
• Ahn NG, Teller DC, Bienkowski MJ, McMullen BA, Lipkin EW, de Haën C . Sedimentation equilibrium analysis of five lipocortin-related phospholipase A2 inhibitors from human placenta. Evidence against a mechanistically relevant association between enzyme and inhibitor . The Journal of Biological Chemistry . 263 . 35 . 18657–63 . Dec 1988 . 10.1016/S0021-9258(18)37335-6 . 2974032 . free .
• Tait JF, Sakata M, McMullen BA, Miao CH, Funakoshi T, Hendrickson LE, Fujikawa K . Placental anticoagulant proteins: isolation and comparative characterization four members of the lipocortin family . Biochemistry . 27 . 17 . 6268–76 . Aug 1988 . 2975506 . 10.1021/bi00417a011 .
• Sjölin C, Stendahl O, Dahlgren C . Calcium-induced translocation of annexins to subcellular organelles of human neutrophils . The Biochemical Journal . 300 (Pt 2) . Pt 2 . 325–30 . Jun 1994 . 8002935 . 1138165 . 10.1042/bj3000325.
• Kojima K, Yamamoto K, Irimura T, Osawa T, Ogawa H, Matsumoto I . Characterization of carbohydrate-binding protein p33/41: relation with annexin IV, molecular basis of the doublet forms (p33 and p41), and modulation of the carbohydrate binding activity by phospholipids . The Journal of Biological Chemistry . 271 . 13 . 7679–85 . Mar 1996 . 8631806 . 10.1074/jbc.271.13.7679 . free .
• Davis AJ, Butt JT, Walker JH, Moss SE, Gawler DJ . The Ca2+-dependent lipid binding domain of P120GAP mediates protein-protein interactions with Ca2+-dependent membrane-binding proteins. Evidence for a direct interaction between annexin VI and P120GAP . The Journal of Biological Chemistry . 271 . 40 . 24333–6 . Oct 1996 . 8798684 . 10.1074/jbc.271.40.24333 . free .
• Satoh A, Takayama E, Kojima K, Ogawa H, Yamori T, Sato S, Kawaguchi T, Tsuruo T, Katsura Y, Kina T, Matsumoto I . Expression of carbohydrate-binding protein p33/41 in human tumor cell lines . Journal of Biochemistry . 119 . 2 . 346–53 . Feb 1996 . 8882729 . 10.1093/oxfordjournals.jbchem.a021246 .
• Satoh A, Takayama E, Kojima K, Ogawa H, Katsura Y, Kina T, Matsumoto I . Characterization of human p33/41 (annexin IV), a Ca2+ dependent carbohydrate-binding protein with monoclonal anti-annexin IV antibodies, AS11 and AS17 . Biological & Pharmaceutical Bulletin . 20 . 3 . 224–9 . Mar 1997 . 9084877 . 10.1248/bpb.20.224 . free .
• Dreier R, Schmid KW, Gerke V, Riehemann K . Differential expression of annexins I, II and IV in human tissues: an immunohistochemical study . Histochemistry and Cell Biology . 110 . 2 . 137–48 . Aug 1998 . 9720986 . 10.1007/s004180050275 . 19597353 .
• Chow A, Davis AJ, Gawler DJ . Identification of a novel protein complex containing annexin VI, Fyn, Pyk2, and the p120(GAP) C2 domain . FEBS Letters . 469 . 1 . 88–92 . Mar 2000 . 10708762 . 10.1016/S0014-5793(00)01252-7 . 21394463 . free .
• Han EK, Tahir SK, Cherian SP, Collins N, Ng SC . Modulation of paclitaxel resistance by annexin IV in human cancer cell lines . British Journal of Cancer . 83 . 1 . 83–8 . Jul 2000 . 10883672 . 2374538 . 10.1054/bjoc.2000.1311 .
• Radau B, Otto A, Müller EC, Westermann P . Protein kinase C alpha-dependent phosphorylation of Golgi proteins . Electrophoresis . 21 . 13 . 2684–7 . Jul 2000 . 10949146 . 10.1002/1522-2683(20000701)21:13<2684::AID-ELPS2684>3.0.CO;2-G . 84800747 .
• Gerner C, Frohwein U, Gotzmann J, Bayer E, Gelbmann D, Bursch W, Schulte-Hermann R . The Fas-induced apoptosis analyzed by high throughput proteome analysis . The Journal of Biological Chemistry . 275 . 50 . 39018–26 . Dec 2000 . 10978337 . 10.1074/jbc.M006495200 . free .
• Tsujii-Hayashi Y, Kitahara M, Yamagaki T, Kojima-Aikawa K, Matsumoto I . A potential endogenous ligand of annexin IV in the exocrine pancreas. Carbohydrate structure of GP-2, a glycosylphosphatidylinositol-anchored glycoprotein of zymogen granule membranes . The Journal of Biological Chemistry . 277 . 49 . 47493–9 . Dec 2002 . 12324456 . 10.1074/jbc.M206572200 . free .

Notes and References

1. Tait JF, Smith C, Frankenberry DA, Miao CH, Adler DA, Disteche CM . Chromosomal mapping of the human annexin IV (ANX4) gene . Genomics . 12 . 2 . 313–8 . Feb 1992 . 1346776 . 10.1016/0888-7543(92)90379-7 .
2. Web site: Entrez Gene: ANXA4 annexin A4.