ANAPC4 explained

Anaphase-promoting complex subunit 4 is an enzyme that in humans is encoded by the ANAPC4 gene.^{[1] [2]}

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known.^[2]

Interactions

ANAPC4 has been shown to interact with ANAPC1,^{[3] [4]} ANAPC5,^[3] CDC27^{[3] [5]} and ANAPC7.^[3]

Further reading

• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery. . Genome Res. . 6 . 9 . 791–806 . 1997 . 8889548 . 10.1101/gr.6.9.791 . free .
• Yu H, Peters JM, King RW . Identification of a cullin homology region in a subunit of the anaphase-promoting complex. . Science . 279 . 5354 . 1219–22 . 1998 . 9469815 . 10.1126/science.279.5354.1219 . etal. 1998Sci...279.1219Y .
• Grossberger R, Gieffers C, Zachariae W . Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. . J. Biol. Chem. . 274 . 20 . 14500–7 . 1999 . 10318877 . 10.1074/jbc.274.20.14500 . etal. free .
• Gieffers C, Peters BH, Kramer ER . Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons. . Proc. Natl. Acad. Sci. U.S.A. . 96 . 20 . 11317–22 . 1999 . 10500174 . 10.1073/pnas.96.20.11317 . 18031 . etal. 1999PNAS...9611317G. free .
• Gmachl M, Gieffers C, Podtelejnikov AV . The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 16 . 8973–8 . 2000 . 10922056 . 10.1073/pnas.97.16.8973 . 16806 . etal. 2000PNAS...97.8973G. free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Vodermaier HC, Gieffers C, Maurer-Stroh S . TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. . Curr. Biol. . 13 . 17 . 1459–68 . 2004 . 12956947 . 10.1016/S0960-9822(03)00581-5 . 5942532 . etal. free . 2003CBio...13.1459V .
• Kraft C, Herzog F, Gieffers C . Mitotic regulation of the human anaphase-promoting complex by phosphorylation. . EMBO J. . 22 . 24 . 6598–609 . 2004 . 14657031 . 10.1093/emboj/cdg627 . 291822 . etal.
• Johnson JM, Castle J, Garrett-Engele P . Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. . Science . 302 . 5653 . 2141–4 . 2004 . 14684825 . 10.1126/science.1090100 . etal. 2003Sci...302.2141J . 10007258 .
• Ota T, Suzuki Y, Nishikawa T . Complete sequencing and characterization of 21,243 full-length human cDNAs. . Nat. Genet. . 36 . 1 . 40–5 . 2004 . 14702039 . 10.1038/ng1285 . etal. free .

Notes and References

1. Tekolf WA, Biddison WE, Aster RD, Shaw S . Two subgroups of HLA Bw44 defined by cell-mediated lympholysis that differ in Bw44 expression on platelets and in patterns of genetic linkage disequilibrium . J Immunol . 129 . 4 . 1474–8 . October 1982 . 10.4049/jimmunol.129.4.1474 . 6180011 .
2. Web site: Entrez Gene: ANAPC4 anaphase promoting complex subunit 4.
3. 10.1016/S0960-9822(03)00581-5 . Vodermaier . Hartmut C . Gieffers . Christian . Maurer-Stroh . Sebastian . Eisenhaber . Frank . Peters . Jan-Michael . September 2003 . TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1 . Curr. Biol. . 13 . 17 . 1459–68 . 0960-9822. 12956947 . 5942532 . free . 2003CBio...13.1459V .
4. 10.1083/jcb.151.4.749 . Sumara . I . Vorlaufer E . Gieffers C . Peters B H . Peters J M . November 2000 . Characterization of vertebrate cohesin complexes and their regulation in prophase . J. Cell Biol. . 151 . 4 . 749–62 . 0021-9525. 11076961 . 2169443 .
5. 10.1073/pnas.97.16.8973 . Gmachl . M . Gieffers C . Podtelejnikov A V . Mann M . Peters J M . August 2000 . The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex . . 97 . 16 . 8973–8 . 0027-8424. 10922056 . 2000PNAS...97.8973G . 16806 . free .