ANAPC2 explained

Anaphase-promoting complex subunit 2 is an enzyme that in humans is encoded by the ANAPC2 gene.^{[1] [2]}

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation.^[2]

Interactions

ANAPC2 has been shown to interact with ANAPC1^{[3] [4]} and ANAPC11.^[5]

Further reading

• Yu H, Peters JM, King RW . Identification of a cullin homology region in a subunit of the anaphase-promoting complex. . Science . 279 . 5354 . 1219–22 . 1998 . 9469815 . 10.1126/science.279.5354.1219 . etal. 1998Sci...279.1219Y .
• Grossberger R, Gieffers C, Zachariae W . Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex. . J. Biol. Chem. . 274 . 20 . 14500–7 . 1999 . 10318877 . 10.1074/jbc.274.20.14500 . etal. free .
• Gieffers C, Peters BH, Kramer ER . Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons. . Proc. Natl. Acad. Sci. U.S.A. . 96 . 20 . 11317–22 . 1999 . 10500174 . 10.1073/pnas.96.20.11317 . 18031 . etal. 1999PNAS...9611317G. free .
• Nagase T, Kikuno R, Ishikawa KI . Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. . DNA Res. . 7 . 1 . 65–73 . 2000 . 10718198 . 10.1093/dnares/7.1.65 . etal. free .
• Gmachl M, Gieffers C, Podtelejnikov AV . The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. . Proc. Natl. Acad. Sci. U.S.A. . 97 . 16 . 8973–8 . 2000 . 10922056 . 10.1073/pnas.97.16.8973 . 16806 . etal. 2000PNAS...97.8973G. free .
• Jarrett CR, Blancato J, Cao T . Human APC2 localization and allelic imbalance. . Cancer Res. . 61 . 21 . 7978–84 . 2001 . 11691822 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . etal. 2002PNAS...9916899M . free .
• Vodermaier HC, Gieffers C, Maurer-Stroh S . TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1. . Curr. Biol. . 13 . 17 . 1459–68 . 2004 . 12956947 . 10.1016/S0960-9822(03)00581-5 . 5942532 . etal. free . 2003CBio...13.1459V .
• Capron A, Serralbo O, Fülöp K . The Arabidopsis anaphase-promoting complex or cyclosome: molecular and genetic characterization of the APC2 subunit. . Plant Cell . 15 . 10 . 2370–82 . 2004 . 14508008 . 10.1105/tpc.013847 . 197302 . etal.
• Kraft C, Herzog F, Gieffers C . Mitotic regulation of the human anaphase-promoting complex by phosphorylation. . EMBO J. . 22 . 24 . 6598–609 . 2004 . 14657031 . 10.1093/emboj/cdg627 . 291822 . etal.
• Humphray SJ, Oliver K, Hunt AR . DNA sequence and analysis of human chromosome 9. . Nature . 429 . 6990 . 369–74 . 2004 . 15164053 . 10.1038/nature02465 . 2734081 . etal. 2004Natur.429..369H .
• Colland F, Jacq X, Trouplin V . Functional proteomics mapping of a human signaling pathway. . Genome Res. . 14 . 7 . 1324–32 . 2004 . 15231748 . 10.1101/gr.2334104 . 442148 . etal.
• Gerhard DS, Wagner L, Feingold EA . The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Rual JF, Venkatesan K, Hao T . Towards a proteome-scale map of the human protein-protein interaction network. . Nature . 437 . 7062 . 1173–8 . 2005 . 16189514 . 10.1038/nature04209 . etal. 2005Natur.437.1173R . 4427026 .
• Dube P, Herzog F, Gieffers C . Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C. . Mol. Cell . 20 . 6 . 867–79 . 2006 . 16364912 . 10.1016/j.molcel.2005.11.008 . etal. free . 11858/00-001M-0000-0010-9405-4 . free .
• Beausoleil SA, Villén J, Gerber SA . A probability-based approach for high-throughput protein phosphorylation analysis and site localization. . Nat. Biotechnol. . 24 . 10 . 1285–92 . 2006 . 16964243 . 10.1038/nbt1240 . 14294292 . etal.

Notes and References

1. Tang Z, Li B, Bharadwaj R, Zhu H, Ozkan E, Hakala K, Deisenhofer J, Yu H . APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase-promoting complex . Mol Biol Cell . 12 . 12 . 3839–51 . December 2001 . 11739784 . 60759 . 10.1091/mbc.12.12.3839.
2. Web site: Entrez Gene: ANAPC2 anaphase promoting complex subunit 2.
3. 10.1016/S0960-9822(03)00581-5 . Vodermaier . Hartmut C . Gieffers . Christian . Maurer-Stroh . Sebastian . Eisenhaber . Frank . Peters . Jan-Michael . September 2003 . TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1 . Curr. Biol. . 13 . 17 . 1459–68 . 0960-9822. 12956947 . 5942532 . free . 2003CBio...13.1459V .
4. 10.1083/jcb.151.4.749 . Sumara . I . Vorlaufer E . Gieffers C . Peters B H . Peters J M . November 2000 . Characterization of vertebrate cohesin complexes and their regulation in prophase . J. Cell Biol. . 151 . 4 . 749–62 . 0021-9525. 11076961 . 2169443 .
5. 10.1016/S1097-2765(00)80482-7 . Ohta . T . Michel J J . Schottelius A J . Xiong Y . April 1999 . ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity . Mol. Cell . 3 . 4 . 535–41 . 1097-2765. 10230407 . 19371828 . free .