TRNA (carboxymethyluridine34-5-O)-methyltransferase explained

TRNA (carboxymethyluridine^34-5-O)-methyltransferase
Ec Number:	2.1.1.229

TRNA (carboxymethyluridine³⁴-5-O)-methyltransferase (ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine³⁴-5-O)-methyltransferase.^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

S-adenosyl-L-methionine + carboxymethyluridine³⁴ in tRNA

\rightleftharpoons S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine³⁴ in tRNA

The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.

Notes and References

1. Fu D, Brophy JA, Chan CT, Atmore KA, Begley U, Paules RS, Dedon PC, Begley TJ, Samson LD . Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival . Molecular and Cellular Biology . 30 . 10 . 2449–59 . May 2010 . 20308323 . 2863699 . 10.1128/MCB.01604-09 .
2. Songe-Møller L, van den Born E, Leihne V, Vågbø CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes PØ, Klungland A . Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding . Molecular and Cellular Biology . 30 . 7 . 1814–27 . April 2010 . 20123966 . 2838068 . 10.1128/MCB.01602-09 .
3. Kalhor HR, Clarke S . Novel methyltransferase for modified uridine residues at the wobble position of tRNA . Molecular and Cellular Biology . 23 . 24 . 9283–92 . December 2003 . 14645538 . 309612 . 10.1128/mcb.23.24.9283-9292.2003 .