ALG1 explained

Chitobiosyldiphosphodolichol beta-mannosyltransferase is an enzyme that is encoded by ALG1^[1] whose structure and function has been conserved from lower to higher organisms.^[2]

Function

The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. The Alg1 mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.^[3] Clinically, the deficiency of ALG1 in humans results in ALG1-CDG, a congenital disorder of glycosylation.^[4]

Further reading

• Couto JR, Huffaker TC, Robbins PW . Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway . J. Biol. Chem. . 259 . 1 . 378–82 . January 1984 . 10.1016/S0021-9258(17)43670-2 . 6368538 . free .
• Gao XD, Nishikawa A, Dean N . Physical interactions between the Alg1, Alg2, and Alg11 mannosyltransferases of the endoplasmic reticulum . Glycobiology . 14 . 6 . 559–70 . June 2004 . 15044395 . 10.1093/glycob/cwh072 . free .
• Otsuki T, Ota T, Nishikawa T . Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries. . DNA Res.. 117–26 . 2007 . 16303743 . 10.1093/dnares/12.2.117 . 12 . 2. etal. free .
• Gerhard DS, Wagner L, Feingold EA . The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC) . Genome Res. . 14 . 10B . 2121–7 . 2004 . 15489334 . 10.1101/gr.2596504 . 528928 . etal.
• Kranz C, Denecke J, Lehle L . Congenital Disorder of Glycosylation Type Ik (CDG-Ik): A Defect of Mannosyltransferase I . Am. J. Hum. Genet. . 74 . 3 . 545–51 . 2004 . 14973782 . 10.1086/382493 . 1182267 . etal.
• Schwarz M, Thiel C, Lübbehusen J . Deficiency of GDP-Man:GlcNAc2-PP-Dolichol Mannosyltransferase Causes Congenital Disorder of Glycosylation Type Ik . Am. J. Hum. Genet. . 74 . 3 . 472–81 . 2004 . 14973778 . 10.1086/382492 . 1182261 . etal.
• Grubenmann CE, Frank CG, Hülsmeier AJ . Deficiency of the first mannosylation step in the N-glycosylation pathway causes congenital disorder of glycosylation type Ik . Hum. Mol. Genet. . 13 . 5 . 535–42 . 2004 . 14709599 . 10.1093/hmg/ddh050 . etal. free . 20.500.11850/51881 . free .
• Clark HF, Gurney AL, Abaya E . The Secreted Protein Discovery Initiative (SPDI), a Large-Scale Effort to Identify Novel Human Secreted and Transmembrane Proteins: A Bioinformatics Assessment . Genome Res. . 13 . 10 . 2265–70 . 2003 . 12975309 . 10.1101/gr.1293003 . 403697 . etal.
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .

External links

• GeneReviews/NCBI/NIH/UW entry on Congenital Disorders of Glycosylation Overview

Notes and References

1. Couto JR, Huffaker TC, Robbins PW . Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway . J. Biol. Chem. . 259 . 1 . 378–82. 1984 . 10.1016/S0021-9258(17)43670-2 . 6368538 . free .
2. Takahashi T, Honda R, Nishikawa Y . Cloning of the human cDNA which can complement the defect of the yeast mannosyltransferase I-deficient mutant alg 1 . Glycobiology . 10 . 3 . 321–7 . Mar 2000 . 10704531 . 10.1093/glycob/10.3.321 .
3. Web site: Entrez Gene: ALG1 asparagine-linked glycosylation 1 homolog (S. cerevisiae, beta-1,4-mannosyltransferase).
4. Web site:
   1. 608540 CONGENITAL DISORDER OF GLYCOSYLATION, TYPE Ik; CDG1K
   . Johns Hopkins University . 2019-05-01.