AKAP8 explained

A-kinase anchor protein 8 is an enzyme that, in humans, is encoded by the AKAP8 gene.^{[1] [2]}

Function

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining it to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is redistributed to distinct locations during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.^[2]

Interactions

AKAP8 has been demonstrated to interact with:

• Cyclin D3^[3]
• DDX5,^[4]
• MCM2,^[5]
• MYCBP,^[6] and
• PRKAR2A.^[7]

Further reading

• Lester LB, Scott JD . Anchoring and scaffold proteins for kinases and phosphatases. . Recent Prog. Horm. Res. . 52 . 409–29; discussion 429–30 . 1997 . 9238861 .
• Michel JJ, Scott JD . AKAP mediated signal transduction. . Annu. Rev. Pharmacol. Toxicol. . 42 . 235–57 . 2002 . 11807172 . 10.1146/annurev.pharmtox.42.083101.135801 .
• Collas P, Le Guellec K, Taskén K . The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis. . J. Cell Biol. . 147 . 6 . 1167–80 . 2000 . 10601332 . 10.1083/jcb.147.6.1167 . 2168084 .
• Steen RL, Cubizolles F, Le Guellec K, Collas P . A kinase-anchoring protein (AKAP)95 recruits human chromosome-associated protein (hCAP)-D2/Eg7 for chromosome condensation in mitotic extract. . J. Cell Biol. . 149 . 3 . 531–6 . 2000 . 10791967 . 10.1083/jcb.149.3.531 . 2174845 .
• Akileswaran L, Taraska JW, Sayer JA . A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase. . J. Biol. Chem. . 276 . 20 . 17448–54 . 2001 . 11279182 . 10.1074/jbc.M101171200 . etal. free .
• Eide T, Carlson C, Taskén KA . Distinct but overlapping domains of AKAP95 are implicated in chromosome condensation and condensin targeting. . EMBO Rep. . 3 . 5 . 426–32 . 2002 . 11964380 . 10.1093/embo-reports/kvf089 . 1084102 . etal.
• Furusawa M, Taira T, Iguchi-Ariga SM, Ariga H . AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex. . J. Biol. Chem. . 277 . 52 . 50885–92 . 2003 . 12414807 . 10.1074/jbc.M206387200 . free .
• Strausberg RL, Feingold EA, Grouse LH . Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. . Proc. Natl. Acad. Sci. U.S.A. . 99 . 26 . 16899–903 . 2003 . 12477932 . 10.1073/pnas.242603899 . 139241 . 2002PNAS...9916899M . etal. free .
• Eide T, Taskén KA, Carlson C . Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication. . J. Biol. Chem. . 278 . 29 . 26750–6 . 2003 . 12740381 . 10.1074/jbc.M300765200 . etal. free .
• Arsenijevic T, Degraef C, Dumont JE . A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95. . Biochem. J. . 378 . Pt 2 . 673–9 . 2004 . 14641107 . 10.1042/BJ20031765 . 1223988 . etal.
• Bouwmeester T, Bauch A, Ruffner H . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway. . Nat. Cell Biol. . 6 . 2 . 97–105 . 2004 . 14743216 . 10.1038/ncb1086 . 11683986 . etal.
• Grimwood J, Gordon LA, Olsen A . The DNA sequence and biology of human chromosome 19. . Nature . 428 . 6982 . 529–35 . 2004 . 15057824 . 10.1038/nature02399 . 2004Natur.428..529G . etal. free .
• Beausoleil SA, Jedrychowski M, Schwartz D . Large-scale characterization of HeLa cell nuclear phosphoproteins. . Proc. Natl. Acad. Sci. U.S.A. . 101 . 33 . 12130–5 . 2004 . 15302935 . 10.1073/pnas.0404720101 . 514446 . 2004PNAS..10112130B . etal. free .
• Kamada S, Kikkawa U, Tsujimoto Y, Hunter T . A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. . Mol. Cell. Biol. . 25 . 21 . 9469–77 . 2005 . 16227597 . 10.1128/MCB.25.21.9469-9477.2005 . 1265837 .
• Beausoleil SA, Villén J, Gerber SA . A probability-based approach for high-throughput protein phosphorylation analysis and site localization. . Nat. Biotechnol. . 24 . 10 . 1285–92 . 2006 . 16964243 . 10.1038/nbt1240 . 14294292 . etal.
• Olsen JV, Blagoev B, Gnad F . Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. . Cell . 127 . 3 . 635–48 . 2006 . 17081983 . 10.1016/j.cell.2006.09.026 . 7827573 . etal. free .

Notes and References

1. Eide T, Coghlan V, Orstavik S, Holsve C, Solberg R, Skalhegg BS, Lamb NJ, Langeberg L, Fernandez A, Scott JD, Jahnsen T, Tasken K . Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95 . Exp Cell Res . 238 . 2 . 305–16 . Mar 1998 . 9473338 . 10.1006/excr.1997.3855 .
2. Web site: Entrez Gene: AKAP8 A kinase (PRKA) anchor protein 8.
3. Mar 2004 . Arsenijevic T, Degraef C, Dumont JE, Roger PP, Pirson I . A novel partner for D-type cyclins: protein kinase A-anchoring protein AKAP95 . Biochem. J. . 378 . Pt 2 . 673–9 . 14641107 . 1223988 . 10.1042/BJ20031765.
4. May 2001 . Akileswaran L, Taraska JW, Sayer JA, Gettemy JM, Coghlan VM . A-kinase-anchoring protein AKAP95 is targeted to the nuclear matrix and associates with p68 RNA helicase . J. Biol. Chem. . 276 . 20 . 17448–54 . 11279182 . 10.1074/jbc.M101171200. free .
5. Jul 2003 . Eide T, Taskén KA, Carlson C, Williams G, Jahnsen T, Taskén K, Collas P . Protein kinase A-anchoring protein AKAP95 interacts with MCM2, a regulator of DNA replication . J. Biol. Chem. . 278 . 29 . 26750–6 . 12740381 . 10.1074/jbc.M300765200. free .
6. Dec 2002 . Furusawa M, Taira T, Iguchi-Ariga SM, Ariga H . AMY-1 interacts with S-AKAP84 and AKAP95 in the cytoplasm and the nucleus, respectively, and inhibits cAMP-dependent protein kinase activity by preventing binding of its catalytic subunit to A-kinase-anchoring protein (AKAP) complex . J. Biol. Chem. . 277 . 52 . 50885–92 . 12414807 . 10.1074/jbc.M206387200. free .
7. Dec 1999 . Collas P, Le Guellec K, Taskén K . The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis . J. Cell Biol. . 147 . 6 . 1167–80 . 10601332 . 2168084 . 10.1083/jcb.147.6.1167.