| Ernest F. Hollings ACE Basin National Wildlife Refuge | |
| Iucn Category: | IV |
| Map: | USA#South Carolina |
| Relief: | 1 |
| Map Width: | 300 |
| Photo Width: | 300 |
| Location: | Beaufort, Charleston, Colleton, Hampton counties, South Carolina, United States |
| Nearest City: | Beaufort, South Carolina |
| Coordinates: | 32.5083°N -80.4667°W |
| Area: | 11815acres |
| Established: | 1990 |
| Governing Body: | U.S. Fish and Wildlife Service |
| Website: | Ernest F. Hollings ACE Basin National Wildlife Refuge |
The Ernest F. Hollings ACE Basin National Wildlife Refuge is a 11815acres portion of the larger ACE Basin area, and the only portions that are federally protected. The wildlife refuge is divided into two units: the Edisto River unit and the Combahee River unit.
The following threatened or endangered species are present in the refuge: wood stork