9beta-pimara-7,15-diene oxidase explained

9beta-pimara-7,15-diene oxidase
Ec Number:	1.14.13.144

9beta-pimara-7,15-diene oxidase (CYP99A3) is an enzyme with systematic name 9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase.^[1] This enzyme catalyses the following chemical reaction

9beta-pimara-7,15-diene + 3 O₂ + 3 NADPH + 3 H⁺

\rightleftharpoons 9beta-pimara-7,15-dien-19-oate + 3 NADP⁺ + 4 H₂O (overall reaction)

(1a) 9beta-pimara-7,15-diene + O₂ + NADPH + H⁺

\rightleftharpoons 9beta-pimara-7,15-dien-19-ol + NADP⁺ + H₂O

(1b) 9beta-pimara-7,15-dien-19-ol + O₂ + NADPH + H⁺

\rightleftharpoons 9beta-pimara-7,15-dien-19-al + NADP⁺ + 2 H₂O

(1c) 9beta-pimara-7,15-dien-19-al + O₂ + NADPH + H⁺

\rightleftharpoons 9beta-pimara-7,15-dien-19-oate + NADP⁺ + H₂O

9beta-pimara-7,15-diene oxidase requires cytochrome P450.

Notes and References

1. Wang Q, Hillwig ML, Peters RJ . CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice . The Plant Journal . 65 . 1 . 87–95 . January 2011 . 21175892 . 3735987 . 10.1111/j.1365-313X.2010.04408.x .