9,12-octadecadienoate 8-hydroperoxide 8R-isomerase explained

9,12-octadecadienoate 8-hydroperoxide 8R-isomerase
Ec Number:	5.4.4.5

9,12-octadecadienoate 8-hydroperoxide 8R-isomerase (5,8-LDS (bifunctional enzyme), 5,8-linoleate diol synthase (bifunctional enzyme), 8-hydroperoxide isomerase, (8R,9Z,12Z)-8-hydroperoxy-9,12-octadecadienoate mutase ((5S,8R,9Z,12Z)-5,8-dihydroxy-9,12-octadecadienoate-forming), PpoA) is an enzyme with systematic name (8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate hydroxymutase ((5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate-forming).^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

\rightleftharpoons (5S,8R,9Z,12Z)-5,8-dihydroxyoctadeca-9,12-dienoate

This enzyme contains heme.

Notes and References

1. Hoffmann I, Jernerén F, Garscha U, Oliw EH . Expression of 5,8-LDS of Aspergillus fumigatus and its dioxygenase domain. A comparison with 7,8-LDS, 10-dioxygenase, and cyclooxygenase . Archives of Biochemistry and Biophysics . 506 . 2 . 216–22 . February 2011 . 21130068 . 10.1016/j.abb.2010.11.022 .
2. Jernerén F, Garscha U, Hoffmann I, Hamberg M, Oliw EH . Reaction mechanism of 5,8-linoleate diol synthase, 10R-dioxygenase, and 8,11-hydroperoxide isomerase of Aspergillus clavatus . Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids . 1801 . 4 . 503–7 . April 2010 . 20045744 . 10.1016/j.bbalip.2009.12.012 .
3. Brodhun F, Göbel C, Hornung E, Feussner I . Identification of PpoA from Aspergillus nidulans as a fusion protein of a fatty acid heme dioxygenase/peroxidase and a cytochrome P450 . The Journal of Biological Chemistry . 284 . 18 . 11792–805 . May 2009 . 19286665 . 2673248 . 10.1074/jbc.M809152200 . free .