6G-fructosyltransferase explained

6G-fructosyltransferase
Ec Number:	2.4.1.243

In enzymology, a 6G-fructosyltransferase is an enzyme that catalyzes the chemical reaction

[1-beta-D-fructofuranosyl-(2->1)-]m⁺1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n⁺1 alpha-D-glucopyranoside

\rightleftharpoons [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n⁺1 beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >\rightleftharpoons 0)

Thus, the two substrates of this enzyme are 1-beta-D-fructofuranosyl-(2->1)-m+1 alpha-D-glucopyranoside]] and 1-beta-D-fructofuranosyl-(2->1)-n+1 alpha-D-glucopyranoside]], whereas its 4 products are 1-beta-D-fructofuranosyl-(2->1)-m alpha-D-glucopyranoside]], 1-beta-D-fructofuranosyl-(2->1)-n+1]], beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >=, and 0.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is 1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase. Other names in common use include fructan:fructan 6G-fructosyltransferase, 1F(1-beta-D-fructofuranosyl)m, sucrose:1F(1-beta-D-fructofuranosyl)_nsucrose, 6G-fructosyltransferase, 6G-FFT, 6G-FT, and 6G-fructotransferase.

References

• Shiomi N . 1981 . Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.) . Carbohydr. Res. . 96 . 281–292 . 10.1016/S0008-6215(00)81878-9 . 2 .
• Shiomi N . 1982 . Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase . Carbohydr. Res. . 106 . 166–169 . 10.1016/S0008-6215(00)80743-0 .
• Shiomi N, Ueno K . 2004 . Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology . J. Appl. Glycosci. . 51 . 177–183 . 10.5458/jag.51.177 . 2 . free .
• Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N . Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis) . The New Phytologist . 165 . 3 . 813–24 . March 2005 . 15720693 . 10.1111/j.1469-8137.2004.01294.x . free .