60S ribosomal protein L32 explained

60S ribosomal protein L32 is a protein that in humans is encoded by the RPL32 gene.^[1]

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L32E family of ribosomal proteins. It is located in the cytoplasm. Although some studies have mapped this gene to 3q13.3-q21, it is believed to map to 3p25-p24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding the same protein have been observed for this gene.^[2]

Further reading

• Wool IG, Chan YL, Glück A . Structure and evolution of mammalian ribosomal proteins . Biochemistry and Cell Biology . 73 . 11–12 . 933–947 . 1996 . 8722009 . 10.1139/o95-101 .
• Young JA, Trowsdale J . A processed pseudogene in an intron of the HLA-DP beta 1 chain gene is a member of the ribosomal protein L32 gene family . Nucleic Acids Research . 13 . 24 . 8883–8891 . December 1985 . 3866218 . 318958 . 10.1093/nar/13.24.8883 .
• Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, Aoki T . 6 . Construction of a human full-length cDNA bank . Gene . 150 . 2 . 243–250 . December 1994 . 7821789 . 10.1016/0378-1119(94)90433-2 .
• Cross SH, Charlton JA, Nan X, Bird AP . Purification of CpG islands using a methylated DNA binding column . Nature Genetics . 6 . 3 . 236–244 . March 1994 . 8012384 . 10.1038/ng0394-236 . 12847618 .
• Bonaldo MF, Lennon G, Soares MB . Normalization and subtraction: two approaches to facilitate gene discovery . Genome Research . 6 . 9 . 791–806 . September 1996 . 8889548 . 10.1101/gr.6.9.791 . free .
• Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC . 6 . A map of 75 human ribosomal protein genes . Genome Research . 8 . 5 . 509–523 . May 1998 . 9582194 . 10.1101/gr.8.5.509 . free .
• Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, de Oliveira PS, Bucher P, Jongeneel CV, O'Hare MJ, Soares F, Brentani RR, Reis LF, de Souza SJ, Simpson AJ . 6 . Shotgun sequencing of the human transcriptome with ORF expressed sequence tags . Proceedings of the National Academy of Sciences of the United States of America . 97 . 7 . 3491–3496 . March 2000 . 10737800 . 16267 . 10.1073/pnas.97.7.3491 . 2000PNAS...97.3491D . free .
• Yoshihama M, Uechi T, Asakawa S, Kawasaki K, Kato S, Higa S, Maeda N, Minoshima S, Tanaka T, Shimizu N, Kenmochi N . 6 . The human ribosomal protein genes: sequencing and comparative analysis of 73 genes . Genome Research . 12 . 3 . 379–390 . March 2002 . 11875025 . 155282 . 10.1101/gr.214202 .
• Odintsova TI, Müller EC, Ivanov AV, Egorov TA, Bienert R, Vladimirov SN, Kostka S, Otto A, Wittmann-Liebold B, Karpova GG . 6 . Characterization and analysis of posttranslational modifications of the human large cytoplasmic ribosomal subunit proteins by mass spectrometry and Edman sequencing . Journal of Protein Chemistry . 22 . 3 . 249–258 . April 2003 . 12962325 . 10.1023/A:1025068419698 . 10710245 .
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G . 6 . A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway . Nature Cell Biology . 6 . 2 . 97–105 . February 2004 . 14743216 . 10.1038/ncb1086 . 11683986 .
• Wan D, Gong Y, Qin W, Zhang P, Li J, Wei L, Zhou X, Li H, Qiu X, Zhong F, He L, Yu J, Yao G, Jiang H, Qian L, Yu Y, Shu H, Chen X, Xu H, Guo M, Pan Z, Chen Y, Ge C, Yang S, Gu J . 6 . Large-scale cDNA transfection screening for genes related to cancer development and progression . Proceedings of the National Academy of Sciences of the United States of America . 101 . 44 . 15724–15729 . November 2004 . 15498874 . 524842 . 10.1073/pnas.0404089101 . 2004PNAS..10115724W . free .
• Baumbusch LO, Myhre S, Langerød A, Bergamaschi A, Geisler SB, Lønning PE, Deppert W, Dornreiter I, Børresen-Dale AL . 6 . Expression of full-length p53 and its isoform Deltap53 in breast carcinomas in relation to mutation status and clinical parameters . Molecular Cancer . 5 . 47 . October 2006 . 17054774 . 1636663 . 10.1186/1476-4598-5-47 . free .
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D . 6 . Large-scale mapping of human protein-protein interactions by mass spectrometry . Molecular Systems Biology . 3 . 1 . 89 . 2007 . 17353931 . 1847948 . 10.1038/msb4100134 .

Notes and References

1. Vorobieva NV, Filipenko ML, Karpova GG, Mertvetsov NP, Graphodatsky AS . Assignment of the L32 ribosomal protein gene (RPL32) to human chromosome 3q13.3→q21 by in situ hybridization . Cytogenetics and Cell Genetics . 77 . 3–4 . 190–191 . Sep 1997 . 9284913 . 10.1159/000134573 .
2. Web site: Entrez Gene: RPL32 ribosomal protein L32.