6-Hydroxy-3-succinoylpyridine 3-monooxygenase explained

6-hydroxy-3-succinoylpyridine 3-monooxygenase
Ec Number:	1.14.13.163

6-hydroxy-3-succinoylpyridine 3-monooxygenase (6-hydroxy-3-succinoylpyridine hydroxylase, hspA (gene), hspB (gene)) is an enzyme with systematic name 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate,NADH:oxygen oxidoreductase (3-hydroxylating, succinate semialdehyde releasing).^{[1] [2]} This enzyme catalyses the following chemical reaction

4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H⁺ + O₂

\rightleftharpoons 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD⁺ + H₂O

6-hydroxy-3-succinoylpyridine 3-monooxygenase catalyses a reaction in the nicotine degradation pathway of Pseudomonas species.

Notes and References

1. Tang H, Wang S, Ma L, Meng X, Deng Z, Zhang D, Ma C, Xu P . A novel gene, encoding 6-hydroxy-3-succinoylpyridine hydroxylase, involved in nicotine degradation by Pseudomonas putida strain S16 . Applied and Environmental Microbiology . 74 . 5 . 1567–74 . March 2008 . 18203859 . 2258644 . 10.1128/AEM.02529-07 . 2008ApEnM..74.1567T .
2. Tang H, Yao Y, Zhang D, Meng X, Wang L, Yu H, Ma L, Xu P . A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida . The Journal of Biological Chemistry . 286 . 45 . 39179–87 . November 2011 . 21949128 . 3234743 . 10.1074/jbc.M111.283929 . free .