6-Carboxyfluorescein Explained

6-Carboxyfluorescein (6-FAM) is a fluorescent dye with an absorption wavelength of 495 nm and an emission wavelength of 517 nm. A carboxyfluorescein molecule is a fluorescein molecule with a carboxyl group added. They are commonly used as a tracer agents. It is used in the sequencing of nucleic acids and in the labeling of nucleotides.

Commercially available FAM is a mixture of two isomers, 5-FAM and 6-FAM, and the correct name is 5(6)-carboxyfluorescein.

The dyes are membrane-impermeant and can be loaded into cells by microinjection or scrape loading.[1] It can be incorporated into liposomes, and allows for the tracking of liposomes as they pass through the body. In addition, carboxyfluorescein has been used to track division of cells.[2] In vascular plants, 5(6)-carboxyfluorescein can be used as a symplastic tracer. It is able to move through the phloem due to its structural similarity to sucrose.[3] It is typically loaded into the leaves in order to gain access to the phloem.[4] [5] This can be done by scraping, cutting, or weakening the leaf’s cuticle with an herbicide.

Popular derivatives for cell tracing purposes are carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and carboxyfluorescein succinimidyl ester (CFSE).

See also

Notes and References

  1. Web site: Molecular Imaging Products Company. 2005-08-26. 5-(and-6)-Carboxyfluorescein (5-(and-6)- FAM,mixed isomer) 100mg. 2006-08-26.
  2. Parish. Christopher. December 1999. Fluorescent dyes for lymphocyte migration and proliferation studies. Immunology and Cell Biology. 77. 6. 499–508. Blackwell Synergy. 10.1046/j.1440-1711.1999.00877.x. 10571670. 2194612. 2006-08-26. subscription.
  3. Schulz. Alexander. Liesche. Johannes. 2013. Modeling the parameters for plasmodesmal sugar filtering in active symplasmic phloem loaders. Frontiers in Plant Science. en. 4. 207. 10.3389/fpls.2013.00207. 23802006. 1664-462X. 3685819. free.
  4. Martens. Helle Juel. Schulz. Alexander. Rademaker. Hanna. Andersen. Signe R.. Binczycki. Piotr. Gao. Chen. Liesche. Johannes. 2019-04-01. Direct Comparison of Leaf Plasmodesma Structure and Function in Relation to Phloem-Loading Type. Plant Physiology. en. 179. 4. 1768–1778. 10.1104/pp.18.01353. 0032-0889. 30723179. 6446768.
  5. Zambryski. P. C.. Hempel. F. D.. Barella. S.. Gisel. A.. 1999-05-01. Temporal and spatial regulation of symplastic trafficking during development in Arabidopsis thaliana apices. Development. en. 126. 9. 1879–1889. 10.1242/dev.126.9.1879. 0950-1991. 10101122. subscription.

This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "6-Carboxyfluorescein".

Except where otherwise indicated, Everything.Explained.Today is © Copyright 2009-2024, A B Cryer, All Rights Reserved. Cookie policy.