50S ribosomal protein L25 explained

50S ribosomal subunit protein L25
Organism:	Escherichia coli (strain K-12 substrain MG1655)
Taxid:	511145
Symbol:	rplY
Entrezgene:	945618
Pdb:	1DFU
Uniprot:	P68919
Chromosome:	genome
Entrezchromosome:	NC_000913.3
Genloc Start:	2282473
Genloc End:	2282843

50S ribosomal protein L25 is a protein that in Escherichia coli is encoded by the gene.

Function

This ribosomal protein is a component of the 50S subunit. The protein binds 5S rRNA to form a stable complex. In turn 5S rRNA binds specifically to three proteins, L25, L18 and L5, forming a separate domain of the bacterial ribosome.^[1] Protein L25 of E. coli is not essential for survival of the cells.^{[2] [3]}

Interactions

Ribosomal protein L25 has been shown to interact with:

• 50S ribosomal protein L16^{[4] [5] [6]}
• 5S ribosomal RNA

Notes and References

1. Chen-Schmeisser U, Garrett RA . A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes . FEBS Letters . 74 . 2 . 287–91 . March 1977 . 321249 . 10.1016/0014-5793(77)80866-1. 2694656 . free .
2. Korepanov AP, Gongadze GM, Garber MB, Court DL, Bubunenko MG . Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli . Journal of Molecular Biology . 366 . 4 . 1199–208 . March 2007 . 17198710 . 1939977 . 10.1016/j.jmb.2006.11.097 .
3. Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H . Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection . Molecular Systems Biology . 2 . 2006.0008 . 2006 . 16738554 . 1681482 . 10.1038/msb4100050 .
4. Harms J, Schluenzen F, Zarivach R, Bashan A, Gat S, Agmon I, Bartels H, Franceschi F, Yonath A . High resolution structure of the large ribosomal subunit from a mesophilic eubacterium . Cell . 107 . 5 . 679–88 . 2001 . 11733066 . 10.1016/S0092-8674(01)00546-3 . 18989228 . free .
5. Selmer M, Dunham CM, Murphy FV, Weixlbaumer A, Petry S, Kelley AC, Weir JR, Ramakrishnan V . Structure of the 70S ribosome complexed with mRNA and tRNA . Science . 313 . 5795 . 1935–42 . September 2006 . 16959973 . 10.1126/science.1131127 . 2006Sci...313.1935S . 9737925 .
6. Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH . Structures of the bacterial ribosome at 3.5 A resolution . Science . 310 . 5749 . 827–34 . November 2005 . 16272117 . 10.1126/science.1117230 . 2005Sci...310..827S . 37382005 .