5-nitrosalicylate dioxygenase explained

5-nitrosalicylate dioxygenase
Ec Number:	1.13.11.64

5-nitrosalicylate dioxygenase (naaB (gene)) is an enzyme with systematic name 5-nitrosalicylate:oxygen 1,2-oxidoreductase (decyclizing).^{[1] [2]} This enzyme catalyses the following chemical reaction

5-nitrosalicylate + O₂

\rightleftharpoons 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite (overall reaction)

(1a) 5-nitrosalicylate + O₂

\rightleftharpoons 4-nitro-6-oxohepta-2,4-dienedioate

(1b) 4-nitro-6-oxohepta-2,4-dienedioate

\rightleftharpoons 2-oxo-3-(5-oxofuran-2-ylidene)propanoate + nitrite (spontaneous reaction)

The enzyme from soil bacterium Bradyrhizobium sp. JS329 participates in 5-nitroanthranilate degradation.

Notes and References

1. Qu Y, Spain JC . Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. strain JS329 . Applied and Environmental Microbiology . 76 . 5 . 1417–22 . March 2010 . 20081004 . 2832354 . 10.1128/AEM.02816-09 .
2. Qu Y, Spain JC . Molecular and biochemical characterization of the 5-nitroanthranilic acid degradation pathway in Bradyrhizobium sp. strain JS329 . Journal of Bacteriology . 193 . 12 . 3057–63 . June 2011 . 21498645 . 3133195 . 10.1128/JB.01188-10 .