5-epiaristolochene 1,3-dihydroxylase explained

5-epiaristolochene 1,3-dihydroxylase
Ec Number:1.14.13.119

5-epiaristolochene 1,3-dihydroxylase (5-epi-aristolochene 1,3-dihydroxylase, EAH) is an enzyme with systematic name 5-epiaristolochene,NADPH:oxygen oxidoreductase (1- and 3-hydroxylating).[1] [2] This enzyme catalyses the following chemical reaction

5-epiaristolochene + 2 NADPH + 2 H+ + 2 O2

\rightleftharpoons

capsidiol + 2 NADP+ + 2 H2O

5-epiaristolochene 1,3-dihydroxylase is a heme-thiolate protein (P-450).

Notes and References

  1. Ralston L, Kwon ST, Schoenbeck M, Ralston J, Schenk DJ, Coates RM, Chappell J . Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum) . Archives of Biochemistry and Biophysics . 393 . 2 . 222–35 . September 2001 . 11556809 . 10.1006/abbi.2001.2483 .
  2. Takahashi S, Zhao Y, O'Maille PE, Greenhagen BT, Noel JP, Coates RM, Chappell J . Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes . The Journal of Biological Chemistry . 280 . 5 . 3686–96 . February 2005 . 15522862 . 2859954 . 10.1074/jbc.M411870200 . free .

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